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Team:DTU-Denmark/Gel preparation and gel electrophoresis
From 2011.igem.org
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== Gel preparation (1% gel) (100 ml of the buffer) == | == Gel preparation (1% gel) (100 ml of the buffer) == | ||
| - | <ol style="list-style-type: | + | <ol style="list-style-type:lower-alpha"> |
<li value="a">100 ml of 1x TBE buffer.</li> | <li value="a">100 ml of 1x TBE buffer.</li> | ||
<li value="b">10 μl of ethidium bomide (10 mg/ml).</li> | <li value="b">10 μl of ethidium bomide (10 mg/ml).</li> | ||
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# Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer. | # Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer. | ||
Gel electrophoresis: | Gel electrophoresis: | ||
| - | <ol style="list-style-type: | + | <ol style="list-style-type:upper-roman"> |
<li value="i">2μl of DNA sample.</li> | <li value="i">2μl of DNA sample.</li> | ||
<li value="ii">3 μl of distilled water.</li> | <li value="ii">3 μl of distilled water.</li> | ||
Revision as of 14:12, 18 September 2011
Gel preparation (1% gel) (100 ml of the buffer)
- 100 ml of 1x TBE buffer.
- 10 μl of ethidium bomide (10 mg/ml).
- 1 g of agarose.
- Assembled gel container.
- Mix buffer with agarose and heat in microwave until the solution is clear
- Add 10μl of ethidium bromide (10mg/ml).
- Pour solution to the gel container and leave it to solidify (30-45 min).
- Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer.
Gel electrophoresis:
- 2μl of DNA sample.
- 3 μl of distilled water.
- 1μl of 6x loading dye.
- 4.2 μl of Gene Ruler DNA ladder mix from Fermentas.
It is recommended to use 7V for each cm of the gel length and to run the gel for 45 min.
