Team:LMU-Munich/Lab Notebook

From 2011.igem.org

(Difference between revisions)
Line 36: Line 36:
<div class="fullbox">
<div class="fullbox">
=Primer=
=Primer=
-
<br>pnikA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
+
 
-
<br>pnikA-S-rev: CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
+
<br><b>pnikA-E,N,X-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
-
<br>prcnA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
+
<br><b>pnikA-S-rev</b> CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
-
<br>prcnA-S-rev: CCGCTACTAGTAcgcaccaagtaagatggcg
+
<br><b>prcnA-E,N,X-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
-
<br>luxCDrev: CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
+
<br><b>prcnA-S-rev</b> CCGCTACTAGTAcgcaccaagtaagatggcg
-
<br>PbrR-M,R-fwd: GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
+
<br><b>luxCDrev</b> CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
-
<br>PbrR-S,A-rev: AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
+
<br><b>PbrR-M,R-fwd</b> GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
-
<br>pbrRT-S,A-rev: agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
+
<br><b>PbrR-S,A-rev</b> AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
-
<br>pbRMut-fwd: tcgtgcgggattctccagggactgtcggactgc
+
<br><b>pbrRT-S,A-rev</b> agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
-
<br>pbrMut-rev: tccgacagtccctggagaatcccgcacgattgggc
+
<br><b>pbRMut-fwd</b> tcgtgcgggattctccagggactgtcggactgc
-
<br>ppbrA-E,N,X-fwd: AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
+
<br><b>pbrMut-rev</b> tccgacagtccctggagaatcccgcacgattgggc
-
<br>ppbrA-S-rev: GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
+
<br><b>ppbrA-E,N,X-fwd</b> AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
-
<br>luxCDfor: GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
+
<br><b>ppbrA-S-rev</b> GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
-
<br>luxBfor: ATGAAATTTGGATTGTATGAAATTTGGATTGT
+
<br><b>luxCDfor</b> GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
-
<br>luxBrev: CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
+
<br><b>luxBfor</b> ATGAAATTTGGATTGTATGAAATTTGGATTGT
-
<br>luxEfor: GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
+
<br><b>luxBrev</b> CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
-
<br>luxErev: CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
+
<br><b>luxEfor</b> GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
-
<br>iscSfor: AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
+
<br><b>luxErev</b> CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
-
<br>icsSrev: agtcactagtattaaccggtctattaatgatgagcccattcg
+
<br><b>iscSfor</b> AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
-
<br>pabAfor: AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
+
<br><b>icsSrev</b> agtcactagtattaaccggtctattaatgatgagcccattcg
-
<br>pabArev: agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
+
<br><b>pabAfor</b> AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
-
<br>aurFfor: AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
+
<br><b>pabArev</b> agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
-
<br>aurFrev: agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
+
<br><b>aurFfor</b> AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
-
<br>ChrBAfor: AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
+
<br><b>aurFrev</b> agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
-
<br>ChrBArev: agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
+
<br><b>ChrBAfor</b> AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
-
<br>melAfor: gatcTCTAGAtgGCCGGC  GCGTGGCTGGTCGGCAAGCCG
+
<br><b>ChrBArev</b> agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
-
<br>melArev: gatcACCGGT  GGCGGACACTATGGCTATTTCTAGC
+
<br><b>melAfor</b> gatcTCTAGAtgGCCGGC  GCGTGGCTGGTCGGCAAGCCG
 +
<br><b>melArev</b> gatcACCGGT  GGCGGACACTATGGCTATTTCTAGC

Revision as of 14:01, 18 September 2011


Week 1

First Primers arrived. Doing lots of PCRs.

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer


pnikA-E,N,X-fwd GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
pnikA-S-rev CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
prcnA-E,N,X-fwd GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
prcnA-S-rev CCGCTACTAGTAcgcaccaagtaagatggcg
luxCDrev CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
PbrR-M,R-fwd GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
PbrR-S,A-rev AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
pbrRT-S,A-rev agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
pbRMut-fwd tcgtgcgggattctccagggactgtcggactgc
pbrMut-rev tccgacagtccctggagaatcccgcacgattgggc
ppbrA-E,N,X-fwd AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
ppbrA-S-rev GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
luxCDfor GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
luxBfor ATGAAATTTGGATTGTATGAAATTTGGATTGT
luxBrev CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
luxEfor GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
luxErev CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
iscSfor AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
icsSrev agtcactagtattaaccggtctattaatgatgagcccattcg
pabAfor AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
pabArev agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
aurFfor AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
aurFrev agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
ChrBAfor AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
ChrBArev agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
melAfor gatcTCTAGAtgGCCGGC GCGTGGCTGGTCGGCAAGCCG
melArev gatcACCGGT GGCGGACACTATGGCTATTTCTAGC


Retrieved from "http://2011.igem.org/Team:LMU-Munich/Lab_Notebook"