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Team:UNAM-Genomics Mexico/Project
From 2011.igem.org
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@NAME Project page for the UNAM-Genomics_Mexico team wiki. | @NAME Project page for the UNAM-Genomics_Mexico team wiki. | ||
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<img class="sub_project" alt="Our Project Image" src="https://static.igem.org/mediawiki/2011/d/dd/GPPU_in_the_lab_500x500.jpg" title="Wet lab"> | <img class="sub_project" alt="Our Project Image" src="https://static.igem.org/mediawiki/2011/d/dd/GPPU_in_the_lab_500x500.jpg" title="Wet lab"> | ||
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| - | <a class="project"><i>Rhizobium etli</i></a> is a bacterial species that can live either freely or in symbiosis with the bean <a class="project"><i>Phaseolus vulgaris</i></a>. | + | <p><a class="project"><i>Rhizobium etli</i></a> is a bacterial species that can live either freely or in symbiosis with the bean <a class="project"><i>Phaseolus vulgaris</i></a>. |
| - | + | In it's symbiotic stage <i>R. etli</i> lives in nodules in the plant's root and has the ability to <a class="project">fixate nitrogen</a> gas as amine molecules. | |
| - | + | Inside the nodule the environment is <a class="project">hypoxigenic</a>. | |
| - | + | The <a class="project">enzymatic production of hydrogen</a> requires a hypoxigenic environment to favor the reactions. | |
| - | + | We want to introduce the metabolic pathway of hydrogen production into <i>R. etli</i> and take advantage of its niche in the hypoxic nodule. | |
| - | + | Our goal is <a class="project">hydrogen</a> production in the <i>Phaseolus vulgaris</i> root.</p> | |
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<div class="descriptive_text"> | <div class="descriptive_text"> | ||
| - | These are the goals in our project: | + | <p>These are the goals in our project:</p> |
<h1 class="project_goals">System design</h1> | <h1 class="project_goals">System design</h1> | ||
<h1 class="project_goals">System insertion into <i>E. coli</i></h1> | <h1 class="project_goals">System insertion into <i>E. coli</i></h1> | ||
<i>Rhizobium etli</i> can't be transformed, and it's phages haven't been studied enough. | <i>Rhizobium etli</i> can't be transformed, and it's phages haven't been studied enough. | ||
| - | However <i>R. etli</i> can receive DNA if conjugated with <i>Escherichia coli</i>. | + | <p>However <i>R. etli</i> can receive DNA if conjugated with <i>Escherichia coli</i>. |
| - | + | To introduce our system into <i>R. etli</i> we must first introduce it into <i>E. coli</i>.</p> | |
<h1 class="project_goals">System conjugation into <i>R. etli</i></h1> | <h1 class="project_goals">System conjugation into <i>R. etli</i></h1> | ||
<h1 class="project_goals">Bean innoculation</h1> | <h1 class="project_goals">Bean innoculation</h1> | ||
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Revision as of 01:53, 13 June 2011
This page is still under construction, please follow this link to view the Biosintetizarte page (in Spanish).
Rhizobium etli is a bacterial species that can live either freely or in symbiosis with the bean Phaseolus vulgaris. In it's symbiotic stage R. etli lives in nodules in the plant's root and has the ability to fixate nitrogen gas as amine molecules. Inside the nodule the environment is hypoxigenic. The enzymatic production of hydrogen requires a hypoxigenic environment to favor the reactions. We want to introduce the metabolic pathway of hydrogen production into R. etli and take advantage of its niche in the hypoxic nodule. Our goal is hydrogen production in the Phaseolus vulgaris root.
These are the goals in our project:
System design
System insertion into E. coli
Rhizobium etli can't be transformed, and it's phages haven't been studied enough.However R. etli can receive DNA if conjugated with Escherichia coli. To introduce our system into R. etli we must first introduce it into E. coli.
