Team:UIUC-Illinois/Notebook

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      <div class="title"><center>About the Team</center></div>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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        <div class="title">Plasmid Integration Protocol (for CRIM plasmids)</div>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:UIUC-Illinois_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:UIUC-Illinois | Team Example]]
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        <div class="desc"><img src="https://static.igem.org/mediawiki/2011/5/52/Illinois_igem_team_s.jpg" alt="Illinois iGEM Team" /></div>
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        <div class="desc">1. Prepare electrocompetent cells that have been transformed with the helper plasmid (pAH57) and that have been grown in 5 mL SOB to an OD of 600nm of ca. 0.6 with ampicillin antibiotic at 30 degC. </div>
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!align="center"|[[Team:UIUC-Illinois|Home]]
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!align="center"|[[Team:UIUC-Illinois/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UIUC-Illinois Official Team Profile]
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!align="center"|[[Team:UIUC-Illinois/Project|Project]]
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!align="center"|[[Team:UIUC-Illinois/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UIUC-Illinois/Modeling|Modeling]]
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!align="center"|[[Team:UIUC-Illinois/Notebook|Notebook]]
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!align="center"|[[Team:UIUC-Illinois/Safety|Safety]]
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!align="center"|[[Team:UIUC-Illinois/Attributions|Attributions]]
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        <div class="desc">2. Perform an electroporation transformation with the BioBrick Compatible Lambda Chromosomal Insertion Plasmid making sure to resuspend the cells in 1 mL of SOC (without ampicillin). Incubate at 37 degC for 1 hour, and then 42 degC for 30 minutes. Spread plate onto a selective agar plate and incubate at 37 degC. </div>
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==Notebook==
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        <div class="title">K617000 Creation</div>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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        <div class="desc">Day 1</div>
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        <div class="desc">The CRIM pAH125 vector was obtained in a pir+ strain.  The culture was streaked from the -80C onto a LB kanamycin 30ug/mL plate.  Plate was incubated 37C for 18 hours.  White colonies resulted.</div>
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        <div class="desc">A single colony was picked from the previous plate and was used to inoculate 6mL of LB kanamycin 30ug/mL.  The culture was then incubated 37C on a tube turner for 16 hours.  A turbid yellow-white culture resulted.</div>
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        <div class="desc">5mL of the culture was pelleted and miniprepped using the Promega mini-prep kit and protocol described in our protocol download.  The resulting concentration was 30ng/uL.</div>
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        <div class="title">The plasmid map of pAH125 is shown below:</div>
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Revision as of 03:28, 29 September 2011

University of Illinois iGEM Team
About the Team
Team Navigation
Notebook
OpenWetWare Notebook

Who We Are
Amanda Chang
"A watched gel never runs"
Plasmid Integration Protocol (for CRIM plasmids)
Illinois iGEM Team
1. Prepare electrocompetent cells that have been transformed with the helper plasmid (pAH57) and that have been grown in 5 mL SOB to an OD of 600nm of ca. 0.6 with ampicillin antibiotic at 30 degC.
2. Perform an electroporation transformation with the BioBrick Compatible Lambda Chromosomal Insertion Plasmid making sure to resuspend the cells in 1 mL of SOC (without ampicillin). Incubate at 37 degC for 1 hour, and then 42 degC for 30 minutes. Spread plate onto a selective agar plate and incubate at 37 degC.
K617000 Creation
Day 1
The CRIM pAH125 vector was obtained in a pir+ strain. The culture was streaked from the -80C onto a LB kanamycin 30ug/mL plate. Plate was incubated 37C for 18 hours. White colonies resulted.
A single colony was picked from the previous plate and was used to inoculate 6mL of LB kanamycin 30ug/mL. The culture was then incubated 37C on a tube turner for 16 hours. A turbid yellow-white culture resulted.
5mL of the culture was pelleted and miniprepped using the Promega mini-prep kit and protocol described in our protocol download. The resulting concentration was 30ng/uL.
The plasmid map of pAH125 is shown below:

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