Team:LMU-Munich/Lab Notebook

From 2011.igem.org

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=Week 7=
=Week 7=
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=Week 8=
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<div class="fullbox">
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=Protocols=
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=Primer=
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<br>pnikA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
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<br>pnikA-S-rev: CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
 +
<br>prcnA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
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<br>prcnA-S-rev: CCGCTACTAGTAcgcaccaagtaagatggcg
 +
<br>luxCDrev: CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
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<br>PbrR-M,R-fwd: GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
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<br>PbrR-S,A-rev: AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
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<br>pbrRT-S,A-rev: agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
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<br>pbRMut-fwd: tcgtgcgggattctccagggactgtcggactgc
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<br>pbrMut-rev: tccgacagtccctggagaatcccgcacgattgggc
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<br>ppbrA-E,N,X-fwd: AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
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<br>ppbrA-S-rev: GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
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<br>luxCDfor: GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
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<br>luxBfor: ATGAAATTTGGATTGTATGAAATTTGGATTGT
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<br>luxBrev: CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
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<br>luxEfor: GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
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<br>luxErev: CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
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<br>iscSfor: AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
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<br>icsSrev: agtcactagtattaaccggtctattaatgatgagcccattcg
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<br>pabAfor: AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
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<br>pabArev: agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
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<br>aurFfor: AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
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<br>aurFrev: agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
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<br>ChrBAfor: AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
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<br>ChrBArev: agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
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<br>melAfor: gatcTCTAGAtgGCCGGC  GCGTGGCTGGTCGGCAAGCCG
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<br>melArev: gatcACCGGT  GGCGGACACTATGGCTATTTCTAGC
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Revision as of 13:57, 18 September 2011


Week 1

First Primers arrived. Doing lots of PCRs.

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer


pnikA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
pnikA-S-rev: CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
prcnA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
prcnA-S-rev: CCGCTACTAGTAcgcaccaagtaagatggcg
luxCDrev: CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
PbrR-M,R-fwd: GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
PbrR-S,A-rev: AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
pbrRT-S,A-rev: agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
pbRMut-fwd: tcgtgcgggattctccagggactgtcggactgc
pbrMut-rev: tccgacagtccctggagaatcccgcacgattgggc
ppbrA-E,N,X-fwd: AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
ppbrA-S-rev: GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
luxCDfor: GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
luxBfor: ATGAAATTTGGATTGTATGAAATTTGGATTGT
luxBrev: CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
luxEfor: GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
luxErev: CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
iscSfor: AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
icsSrev: agtcactagtattaaccggtctattaatgatgagcccattcg
pabAfor: AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
pabArev: agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
aurFfor: AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
aurFrev: agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
ChrBAfor: AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
ChrBArev: agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
melAfor: gatcTCTAGAtgGCCGGC GCGTGGCTGGTCGGCAAGCCG
melArev: gatcACCGGT GGCGGACACTATGGCTATTTCTAGC


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