"
Team:DTU-Denmark-2
From 2011.igem.org
| Line 70: | Line 70: | ||
<td width="600px" height="100%" valign="top" > | <td width="600px" height="100%" valign="top" > | ||
<font color="#990000" face="arial" size="6" > | <font color="#990000" face="arial" size="6" > | ||
| - | <p align=" | + | <p align="left"> |
| - | <b> | + | <b> Why Plug 'n' Play with DNA?</b><br> |
| + | <br> | ||
</font> | </font> | ||
| - | < | + | <p align="justify"> |
| + | We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans. | ||
| + | </p> | ||
<div class="flash"> | <div class="flash"> | ||
| Line 80: | Line 83: | ||
<object width="600" height="500" data="https://static.igem.org/mediawiki/2011/9/99/Overveiw_flash_2.swf"><param NAME="wmode" VALUE="transparent"></object> | <object width="600" height="500" data="https://static.igem.org/mediawiki/2011/9/99/Overveiw_flash_2.swf"><param NAME="wmode" VALUE="transparent"></object> | ||
</div> | </div> | ||
| - | |||
</p> | </p> | ||
| + | |||
| + | |||
<td width="182px" height="100%" valign="top" class="box"> | <td width="182px" height="100%" valign="top" class="box"> | ||
| Line 148: | Line 152: | ||
<tr height="25px"> | <tr height="25px"> | ||
| - | <td width=" | + | <td width="976px" valign="center" align="center"> |
<font color="#990000" face="arial" size="6"> | <font color="#990000" face="arial" size="6"> | ||
<b> Sponsored by</b><br><br> | <b> Sponsored by</b><br><br> | ||
Revision as of 14:53, 16 September 2011
|
Why Plug 'n' Play with DNA? We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans. |
|
Sponsored by
 
 
Comments or questions to the team? Please Email us |
