Team:Cornell/Week 1

(Difference between revisions)

Revision as of 01:05, 16 September 2011

Results | Protocol | Notebook | Parts Submitted

June 5th - June 11th

CU GEM Bootcamp Training Session (Day 1)

Part I

1. PCR setup

2. Run an agarose gel and gel purify the samples using Qiagen Kit.

3. Quantify the samples using NanoDrop.

4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.

5. PCR clean up the samples and quantify using NanoDrop.

Part II

1. Miniprep the overnight culture using Qiagen Kit.

2. Quantify the samples using NanoDrop.

3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.

4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.

5. Run the samples on the agarose gel.

6. Cut out the band of right size, gel purify, and quantify with NanoDrop.

Weill Hall labspace introduction by Dr.Archer

CU iGEM bootcamp training session (day 2)

1. Setup ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.

2. Desalt the ligation samples on a membrane.

3. Electroporate the samples in the electrocompetent cells.

4. Shake the cells in 37°C shaker for hour.

5. Plate the cells onto the plate with appropriate antibiotic. Incubate it overnight at 37°C.

CU iGEM bootcamp training session (day 3)

1. Check the colonies on the plates.

Team meeting

@@ Line 11: / Line 11: @@
 ==Monday, June 6==
-==Tuesday==
+==Tuesday, June 7==
-:'''CU iGEM bootcamp training session (day 1)'''
+:'''CU GEM Bootcamp Training Session (Day 1)'''
 ::'''Part I'''
 :::1. PCR setup
-:::2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
+:::2. Run an agarose gel and gel purify the samples using Qiagen Kit.
-:::3. Quantify the samples using nanodrop.
+:::3. Quantify the samples using NanoDrop.
-:::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
+:::4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.
-:::5. PCR clean up the samples and quantify using nanodrop.
+:::5. PCR clean up the samples and quantify using NanoDrop.
 ::'''Part II'''
-:::1. Miniprepping the overnight culture using Qaigen Kit.
+:::1. Miniprep the overnight culture using Qiagen Kit.
-:::2. Quantify the samples using nanodrop.
+:::2. Quantify the samples using NanoDrop.
-:::3. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
+:::3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.
-:::4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes.
+:::4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.
 :::5. Run the samples on the agarose gel.
-:::6. Cut out the band of right size, gel purify and quantify with nanodrop.
+:::6. Cut out the band of right size, gel purify, and quantify with NanoDrop.
 ==Wednesday==