"
Team:Kyoto/Notebook
From 2011.igem.org
Grassfield (Talk | contribs) (→Lab Note) |
Grassfield (Talk | contribs) (→Protocol) |
||
| Line 214: | Line 214: | ||
<html><a name="protocol"></a></html> | <html><a name="protocol"></a></html> | ||
== Protocol == | == Protocol == | ||
| + | |||
| + | ::{| class="wikitable" | ||
| + | |+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila] | ||
| + | ! width="300" |Materials | ||
| + | ! width="300" |Methods | ||
| + | |- | ||
| + | | | ||
| + | * water 500mL | ||
| + | * dry yeast 20g | ||
| + | * corn flour 45g | ||
| + | * glucose 50g | ||
| + | * agarose 3.5~5.0g | ||
| + | * propionic acid 1.5mL | ||
| + | | | ||
| + | # Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it. | ||
| + | # Stir corn flour and glucose with the remaining water. | ||
| + | # Stir 1 and 2, then autoclave it again. | ||
| + | # after autoclave, add propionic acid into it.■ | ||
| + | |} | ||
Revision as of 11:33, 15 September 2011
Lab Note
Week1: Monday 1st - Sunday 7th August
Monday
行った実験名：
使った試薬名、容量：
用いた機械：
行った人：
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week2: Monday 8th - Sunday 14th August
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week3: Monday 15th - Sunday 21th August
Monday
Tuesday
Wednesday
Thursday
Thursday
Friday
Saturday
Sunday
Week4: Monday 22th - Sunday 28th August
Monday
Tuesday
Wednesday
Thursday
Luminescence(Kusaba, Terada, Hara):ハエの走行性実験① ♂、紫外線×2回、緑×2回 ♀、紫外線×2回、緑×2回
Friday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回
Saturday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤×2回、青×2回 ♀、赤×2回、青×2回
Sunday
Week5: Monday 29th August - Sunday 4th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week6: Monday 5th September - Sunday 11th September
Monday
Tuesday
Luminescence(Kusaba, Hara):ハエの走行性実験②（②は改良版） ♂、緑×2回 ♀、青×1回
Wednesday
Luminescence(Hara):ハエの走行性実験② ♂、青×2回 ♀、緑×2回、青×1回
Thursday
Luminescence(Hara):ハエの走行性実験② ♂、紫外線×3回 ♀、紫外線×3回
Friday
Saturday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、青×2回、赤×2回 ♀、青×2回、赤×2回
Sunday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、紫外線×2回、
Week7: Monday 12th September - Sunday 18th September
Monday
Luminescence:大腸菌の形質転換(Hashiya) ハエの走行性実験②(Kusaba, Hara)
Tuesday
Luminescence:大腸菌はじめて光る。しかし光量は少ない。
Wednesday
Thursday
Friday
Saturday
Sunday
Week8: Monday 19th September - Sunday 25th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week9: Monday 26th September - Sunday 2nd October
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Protocol
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila] Materials Methods - water 500mL
- dry yeast 20g
- corn flour 45g
- glucose 50g
- agarose 3.5~5.0g
- propionic acid 1.5mL
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid into it.■
