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Revision as of 08:26, 15 September 2011
Plug ānā Play biobricks
We think that iGEM should be about combining biobricks in any thinkable way fast and easy, leaving more time for characterization of parts and focusing on the actual project. Therefore we have developed a novel assembly standard called Plug'n'Play with DNA based on uracil excision based cloning. The concept of our assembly standard is that iGEM teams receive a kit containing ready to use PCR products of every single biobrick. This allow for the teams to select the biobricks they need mix them in a USER reaction incubate it and 70 min later, competent E. coli cells can be transformed. The use of preproduced PCR products may be to unconventional for some people and therefore the kit also contains what we call a back-up plasmid containing all parts in the kit, so if you use up all your PCR product or you prefer to run our own PCR you simply use the back-up plasmids. The Plug'n'Play with DNA kit so far contains 49 biobricks and 21 plasmids for mammalian cells and Aspergilli.
Data For Our Favorite Parts
1.Main page - DMKP-P6 promoter, BBa_K678000, The specific activity of the constitutive promoter was assayed and compared to a strong promoter the promoter appears to be of medium strength.
2.Main page - PalcA promoter, BBa_K678001 . .
3.Main page - Device for expression of GFP in mammalian cells, BBa_K678002, This device was used to transfect the mammalian cell line U-2 OS and as expected resulted in the localization of GFP to the peroxisomes.
Data For Pre-existing parts
1.Experience - CMV promoter, BBa_J52034
We've also characterized the following parts
1.Main page - BBa_K678XXX
