Revision as of 02:04, 15 September 2011

June

Restriction, Ligation, and Transformation Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
Restricted destination plasmid pSB1C3 with EcoR1 and Pst1

Picked cells from June 21st transformation

Minipreped Cells picked on June 25th, 2011

Restriction digest and ran on gel Lumazine - dt construct successfully inserted into pSB1C3 Glycerol stocked.

Add Gel Picture

Performed antibiotic assay on lumazine - dt in pSB1C3 Samples from one colony grew in Kanamicyn; this stock was discarded

PCR: PCR'd out RFP and lumazine

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 . [[Team:Lethbridge/Notebook|Restriction]]
-#* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
+* Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
-#* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
+* Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
 . [[Team: Lethbridge/Notebook|Ligation]]
-#* Ligated lumazine synthase-dt into destination plasmid pSB1C3
+* Ligated lumazine synthase-dt into destination plasmid pSB1C3
-#* Ligated pBAD-RBS into destination plasmid pSB1C3
+* Ligated pBAD-RBS into destination plasmid pSB1C3
 . [[Team:Lethbridge/Notebook|Transformation]]
-#* Transformed lumazine synthase - dt in pSB1C3 into DH5&prop;
+* Transformed lumazine synthase - dt in pSB1C3 into DH5&prop;
-#* Transformed pBAD-RBS in pSB1C3 into DH5&prop;
+* Transformed pBAD-RBS in pSB1C3 into DH5&prop;
 ==June 24th, 2011==