"
Team:WarrenCIndpls IN-HS/Notebook
From 2011.igem.org
(→May 19th: Research on Parts) |
(→May 26th: Primer Design) |
||
| Line 47: | Line 47: | ||
==May 26th: Primer Design== | ==May 26th: Primer Design== | ||
| - | 1st Forward Primer contains an overhang to attach the biobrick to the plasmid, two restriction enzyme sites ( | + | Primers- the 3' section must be complementary to the DNA template, the 5' end may have additional, noncomplimentary base sequences to add restriction enzyme sites, and the 3' sections should not be complimentary to each other (increases risk of primer-dimers forming and inhibiting amplification); forward primers extend from start codon to stop codon while reverse primers work from stop codon to start codon |
| + | |||
| + | 1st Forward Primer contains an overhang to attach the biobrick to the plasmid, two restriction enzyme sites (for cutting the sequnce out), and a primer for the constructin of a new strand | ||
2nd Forward Primer contains a primer for contructing a new strand to connect the translational unit to the biobrick | 2nd Forward Primer contains a primer for contructing a new strand to connect the translational unit to the biobrick | ||
Reverse Primer contains an overhang to attach the biobrick to the multi-cloning site (on the plasmid), the other two restriction enzymes sites, and a primer for contructiong a new strand | Reverse Primer contains an overhang to attach the biobrick to the multi-cloning site (on the plasmid), the other two restriction enzymes sites, and a primer for contructiong a new strand | ||
Revision as of 13:52, 9 June 2011
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
|
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:WarrenCIndpls IN-HS team.png Your team picture |
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy; the amount of protein synthesized from mRNA is dependent on the strength of the Kozak sequence
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors
Terminator Sequence- signals the end of transcriptoin to RNA polymerase
Vector- Plasmid
ORI (Origin of Replication)
Selection Markers
-Ura3 is a selection marker for yeast
-Ampicillin Resistance is a selection marker for bacteria based antibiotic resistance
May 26th: Primer Design
Primers- the 3' section must be complementary to the DNA template, the 5' end may have additional, noncomplimentary base sequences to add restriction enzyme sites, and the 3' sections should not be complimentary to each other (increases risk of primer-dimers forming and inhibiting amplification); forward primers extend from start codon to stop codon while reverse primers work from stop codon to start codon
1st Forward Primer contains an overhang to attach the biobrick to the plasmid, two restriction enzyme sites (for cutting the sequnce out), and a primer for the constructin of a new strand
2nd Forward Primer contains a primer for contructing a new strand to connect the translational unit to the biobrick
Reverse Primer contains an overhang to attach the biobrick to the multi-cloning site (on the plasmid), the other two restriction enzymes sites, and a primer for contructiong a new strand
