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	==Notebook==		==Notebook==
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	===Week 2===		===Week 2===
		+	Performed PCR on esp and rsaA genes and run on gel.  Results showed successful amplification.

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Revision as of 22:08, 9 June 2011

Notebook

Week 1

PCR primers designed according to Biobrick specifications and ordered for rsaA C-terminal and WT esp. Because of the discrepancy in GC content between Staphylococcus epidermidis and Caulobacter, we also optimized the esp sequence for Caulobacter for later synthesis with a higher resulting GC content.

Prepared competent cells of E. coli Top10. (Protocol)

Streaked plates of Caulobacter, Staphylococcus aureus, and S. epidermidis.

Template DNA for PCR prepared from colonies using [http://www.bioventures.com/products.php?cid=10005| GeneReleaser].

Week 2

Performed PCR on esp and rsaA genes and run on gel. Results showed successful amplification.