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Team:EPF-Lausanne/Todo
From 2011.igem.org
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=== Protocols === | === Protocols === | ||
| - | For somebody who's made some cell cultures, please provide a sentence or two on how they're done, in the Miniprep protocol: | + | <s>For somebody who's made some cell cultures, please provide a sentence or two on how they're done, in the Miniprep protocol:</s> |
| - | * Describe cell cultures in [[Team:EPF-Lausanne/Protocols/Miniprep|miniprep protocol]] | + | * <s>Describe cell cultures in [[Team:EPF-Lausanne/Protocols/Miniprep|miniprep protocol]]</s> |
=== General === | === General === | ||
Revision as of 23:44, 9 June 2011
Todo
Contents |
Microfluidic Chips
- Continue alignment training
Preparing the parts
- Investigate failure of lysis device sequencing.
Next steps:
-
Make competent cells -
Test competent cells~10^5CFU/µg not very good -
Transform in E. Coli. - Glycerol Stocks
-
Plasmid preps - Sequence the lysis cassette
Assembly
-
Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
Protocols
For somebody who's made some cell cultures, please provide a sentence or two on how they're done, in the Miniprep protocol:
-
Describe cell cultures in miniprep protocol
General
- Write a new protocol!
- Write-up team presentation
Clean rooms
- Order lab notebook
- Order storage box
