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Team:UC Davis/Notebook/Week 12
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Today marked the beginning of our characterization of R0010 mutants. We plan on characterizing six mutants at a time by running many 96-well plates (8 rows, 12 columns) through the Tecan. We will have six rows for mutants, one row for wild type, and a final row for our controls. Each plate will have a constant arabinose level in all wells (except the controls) and will have 4 levels of IPTG induction across each row. Not only will we be able to get a lot of data about how each mutant responds to various repressor concentrations, but we will determine a good range of arabinose and IPTG levels to test over in the upcoming weeks. | Today marked the beginning of our characterization of R0010 mutants. We plan on characterizing six mutants at a time by running many 96-well plates (8 rows, 12 columns) through the Tecan. We will have six rows for mutants, one row for wild type, and a final row for our controls. Each plate will have a constant arabinose level in all wells (except the controls) and will have 4 levels of IPTG induction across each row. Not only will we be able to get a lot of data about how each mutant responds to various repressor concentrations, but we will determine a good range of arabinose and IPTG levels to test over in the upcoming weeks. | ||
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The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM. | The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM. | ||
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Revision as of 22:51, 12 September 2011
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View our judging criteria for iGEM 2011 here.--Monday 8/29/11--
--Wednesday 8/31/11--
Today marked the beginning of our characterization of R0010 mutants. We plan on characterizing six mutants at a time by running many 96-well plates (8 rows, 12 columns) through the Tecan. We will have six rows for mutants, one row for wild type, and a final row for our controls. Each plate will have a constant arabinose level in all wells (except the controls) and will have 4 levels of IPTG induction across each row. Not only will we be able to get a lot of data about how each mutant responds to various repressor concentrations, but we will determine a good range of arabinose and IPTG levels to test over in the upcoming weeks.
The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with are 0 mM, 0.25 mM, 0.5 mM, and 1 mM.
