Team:BU Wellesley Software/Puppetshow
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Lab protocols often require careful and precise execution of many individual steps to achieve the desired result. Slight inaccuracies and miscalculations can lead to invalid conclusions. We understand this crucial need to develop a less error prone workflow that will allow us to execute complicated and repetitive lab protocols in a more systematic and infallible matter. Our solution to this problem is Puppeteer, a high-level protocol specifying language that will allow users to construct protocols using our library of Common Human Robot Instruction Set (CHRIS). Using this language, users will be able to create protocols and save them into a Protocol Repository that we have created. This will allow synthetic biologists to share their work among themselves, therefore promoting collaboration within the synthetic biology community. | Lab protocols often require careful and precise execution of many individual steps to achieve the desired result. Slight inaccuracies and miscalculations can lead to invalid conclusions. We understand this crucial need to develop a less error prone workflow that will allow us to execute complicated and repetitive lab protocols in a more systematic and infallible matter. Our solution to this problem is Puppeteer, a high-level protocol specifying language that will allow users to construct protocols using our library of Common Human Robot Instruction Set (CHRIS). Using this language, users will be able to create protocols and save them into a Protocol Repository that we have created. This will allow synthetic biologists to share their work among themselves, therefore promoting collaboration within the synthetic biology community. |
Revision as of 15:22, 12 September 2011
Puppeteer Overview
Lab protocols often require careful and precise execution of many individual steps to achieve the desired result. Slight inaccuracies and miscalculations can lead to invalid conclusions. We understand this crucial need to develop a less error prone workflow that will allow us to execute complicated and repetitive lab protocols in a more systematic and infallible matter. Our solution to this problem is Puppeteer, a high-level protocol specifying language that will allow users to construct protocols using our library of Common Human Robot Instruction Set (CHRIS). Using this language, users will be able to create protocols and save them into a Protocol Repository that we have created. This will allow synthetic biologists to share their work among themselves, therefore promoting collaboration within the synthetic biology community.To aid the creation of new protocols using Puppeteer, we have also created a GUI front-end for our language called PuppetShow. Within PuppetShow, users can create protocols in the editor panel and execute the protocol they just wrote with the click of a button. Given that the user’s computer is hooked up to a compatible robot, resource allocation of liquids and plates will happen automatically and a report will be generated containing a resource report, an output report, instructions for the deck setup, and a pipette verification report. For now, we have the software bridge written for the Evoware 150 API and support all major robot commands through this bridge. So far, we have created Restriction Digest and Ligation protocol using PuppetShow and successfully verified their execution on our robot.
Using this tool we have created, we aim to improve accuracy and save time. A protocol only needs to be written once and can be run repeatedly with the click of a button. Using PuppetShow, synthetic biologists can create and run protocols without worrying about any low level details or error.