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Commons

Minipreps

Investigators: Sandra

Because of the positive testdigest, a miniprep of the PR+GFP was performed. Samples were send for sequencing.

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep of PR+lovtap-notgate domain

Investigators: Sandra

Miniprep of:

• 39A
• 39B
• 40A
• 40B
• 41A
• 41B
• 42A
• 42B
• 44A
• 44B

39=PR1, 40=PR2, 41=PR3, 42=PR4, 44=PR6.

2A-assembly: Digestion

Investigators: Sandra

Digestion of the samples from miniprep (10 samples) and digestion of RFP (S1b, S2b).

PR with Lov-Notgate domain:

• 39A
• 39B
• 40A
• 40B
• 41A
• 41B
• 42A
• 42B
• 44A
• 44B

digested with NehI and PstI.

RFP:

• S1b
• S2b

digested with SpeI and PstI.

2A-assembly: Ligation

Ligation of RFP with 39-44.

• 39A-S1b
• 39A-S2b

• 39B-S1b
• 39B-S2b

• 40A-S1b
• 40A-S2b

• 40B-S1b
• 40B-S2b

• 41A-S1b
• 41A-S2b

• 41B-S1b
• 41B-S2b

• 42A-S1b
• 42A-S2b

• 42B-S1b
• 42B-S2b

• 44A-S1b
• 44A-S2b

• 44B-S1b
• 44B-S2b

Trafo

Trafo of the ligated samples on plates with Cm.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME