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Team:UST-Beijing/Notebook
From 2011.igem.org
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<p><img src="https://static.igem.org/mediawiki/2011/c/c4/PR_for_pSG5.jpg" width="313" height="89" /><img src="https://static.igem.org/mediawiki/2011/f/fb/PSG5.JPG" width="321" height="231" /><img src="https://static.igem.org/mediawiki/2011/3/33/PSG5PR.jpg" width="321" height="244" /></p> | <p><img src="https://static.igem.org/mediawiki/2011/c/c4/PR_for_pSG5.jpg" width="313" height="89" /><img src="https://static.igem.org/mediawiki/2011/f/fb/PSG5.JPG" width="321" height="231" /><img src="https://static.igem.org/mediawiki/2011/3/33/PSG5PR.jpg" width="321" height="244" /></p> | ||
| - | <p>PR + pSG5 = pSG5/PR | + | <p>PR + pSG5 = pSG5/PR <br /> |
| - | Cut PR w/EcoRI & BamH I | + | Cut PR w/EcoRI & BamH I<br /> |
| - | Cut pSG5 w/EcoRI & BamH I | + | Cut pSG5 w/EcoRI & BamH I<br /> |
| - | Combine the insert and vector with T4 ligase</p> | + | Combine the insert and vector with T4 ligase</p> |
<p> </p> | <p> </p> | ||
Revision as of 02:41, 12 September 2011
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
Project 2.
1.1：The construction of pSB1AC3/PR
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PR + pSB1AC3 = pSB1AC3/PR (new part)
Cut PR w/EcoRI & SpeI
Cut pSB1AC3 w/EcoRI & SpeI
Combine the insert and vector with T4 ligase
1.2: Gel electrophoresis
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1: wide range maker
2、3、4、5：the constructed plasmid cutted with SpeI and EcoRI restriction enzymes |
1.3: DNA sequencing
The result of DNA sequencing demonstrates that there is no mutation in the new part by comparing to the original sequence.
The result of DNA sequencing demonstrates that there is no mutation in the new part by comparing to the original sequence.
2.1 The construction of pSG5/PR which is used for eukaryotic expression
PR + pSG5 = pSG5/PR
Cut PR w/EcoRI & BamH I
Cut pSG5 w/EcoRI & BamH I
Combine the insert and vector with T4 ligase
Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
