Sensor: Week 2 May 23-27

From 2011.igem.org

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==Tuesday==
==Tuesday==
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[[File:Jamie_research.jpg|thumb|right|400px|Jamie says: "No more research. To the lab!"]]
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==Wednesday==
==Wednesday==
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[[File:Everyone_lab_waiting_safe.jpg|right|thumb|400px|Jamie says: "D'oh! No one said anything about WAITING!"]]
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!Assembly Step
!Assembly Step

Revision as of 15:49, 8 June 2011

Contents

Monday

Assembly Step Parts Involved in
Assembly Step
PCR J23113
J23105
J23118
J23100

Tuesday

Jamie says: "No more research. To the lab!"
Assembly Step Parts Involved in Assembly Step
PCR Purification J23113
J23105
J23118
J23100
Miniprep B0051
Restriction Digest Inserts using EcoRI and SpeI: J23113
J23105
J23118
J23100
B0034+Mcherry
Vectors using EcoRI and XbaI: B0034+C0051
B0015
Ligation J23113 and B0034+C0051
J23105 and B0034+C0051
J23118 and B0034+C0051
J23100 and B0034+C0051
B0034+Mcherry and B0015
Transformation/Plating The above ligations were transformed into Escherichia
coli cells and plated onto Amp resistant plates.
PCR B0030
B0031
B0032
B0033

Wednesday

Jamie says: "D'oh! No one said anything about WAITING!"
Assembly Step Parts Involved with Assembly Step
Colony PCR B0034+Mcherry+B0015 (Kan resistance)
B0034+Mcherry+B0015 (Amp resistance)
PCR Purification B0030
B0031
B0032
B0033
Restriction Digest Inserts using EcoRI and SpeI: B0030
B0031
B0032
B0033
Vector using EcoRI and XbaI: C0051
Ligation B0030+C0051
B0031+C0051
B0032+C0051
B0033+C0051
Transformation/Plating The above ligations were transformed
into Escherichia coli cells and plated onto
Amp resistant plates.

Thursday

Assembly Step Part Involved with Assembly Step
Miniprep for
Sequencing
B0034+Mcherry+B0015
J23113+B0034+C0051
J23105+B0034+C0051
PCR B0034+Mcherry+B0015
Restriction Digest Insert using EcoRI and SpeI: B0034+Mcherry+B0015
Vectors using EcoRI and XbaI: J23113+B0034+C0051
J23105+B0034+C0051
Ligation B0034+Mcherry+B0015 and J23113+B0034+C0051
B0034+Mcherry+B0015 and J23105+B0034+C0051
Transformation/Plating The above ligations were transformed into Escherichia
coli cells and plated onto Amp resistant plates.

Note: The minipreps in green font indicate the sequencing proved the assembly worked.

Friday

The following assemblies have been confirmed and named for the registry.

Assembly Registry Name
B0034+mCherry+B0015
J23113+B0034+C0051
J23105+B0034+C0051
K648000
K648001
K648002





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