Team:Michigan/Protocols

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[[Media: quick-ligase.pdf | Using quick ligase]]
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[[Media: T4-ligase.pdf | Using T4 ligase]]

Revision as of 20:53, 7 June 2011


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Home Team Project BioBricks Human Practices Notebook Safety Attributions

Protocols

Basic protocols ::: These protocols are necessary for all lab operations.

Autoclave usage

OD600 optimization


Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.

Gel electrophoresis

Using the gel viewer


Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Production of electrocompetent cells

Electroporation of electrocompetent cells

Production of standard competent cells

Quick Transformation (QT) of standard competent cells


Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.

Using quick ligase

Using T4 ligase