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Team:Yale/Notebook/Week3
From 2011.igem.org
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''Tuesday'' | ''Tuesday'' | ||
- Darren arrives | - Darren arrives | ||
| + | |||
- Discussion of R. inquisitor | - Discussion of R. inquisitor | ||
''Wednesday'' | ''Wednesday'' | ||
- Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid | - Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid | ||
| + | |||
- Modelling/homology study for RiAFP | - Modelling/homology study for RiAFP | ||
| + | |||
- Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize | - Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize | ||
| + | |||
- Emailed companies for sponsorship for gene synthesis | - Emailed companies for sponsorship for gene synthesis | ||
| + | |||
- Emailed other iGEM teams for suggestion | - Emailed other iGEM teams for suggestion | ||
''Thursday'' | ''Thursday'' | ||
- Found protocol for Origami cell lines at Xiong lab | - Found protocol for Origami cell lines at Xiong lab | ||
| + | |||
- Designed gene on IDT website | - Designed gene on IDT website | ||
''Friday'' | ''Friday'' | ||
- Asking about TEV, GFP, His | - Asking about TEV, GFP, His | ||
| + | |||
- Ordered gene | - Ordered gene | ||
| + | |||
- Contacted Israeli lab about Plasmids | - Contacted Israeli lab about Plasmids | ||
''Saturday'' | ''Saturday'' | ||
- Miniprep, Nanodrop | - Miniprep, Nanodrop | ||
| + | |||
- Cultured 2 colonies in 100 mL flasks | - Cultured 2 colonies in 100 mL flasks | ||
| + | |||
- Warren worked on primer design | - Warren worked on primer design | ||
Revision as of 20:28, 7 June 2011
Notebook: Week 3
Monday - Food
Tuesday - Darren arrives
- Discussion of R. inquisitor
Wednesday - Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid
- Modelling/homology study for RiAFP
- Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize
- Emailed companies for sponsorship for gene synthesis
- Emailed other iGEM teams for suggestion
Thursday - Found protocol for Origami cell lines at Xiong lab
- Designed gene on IDT website
Friday - Asking about TEV, GFP, His
- Ordered gene
- Contacted Israeli lab about Plasmids
Saturday - Miniprep, Nanodrop
- Cultured 2 colonies in 100 mL flasks
- Warren worked on primer design
Sunday - 1/40 dilution in 1 liter
