Team:Wageningen UR/Project/Aftermath
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- | After several months of running with our two separate | + | After several months of running with our two separate projects we noticed that our divided attention was taxing our ability to succesfully complete two projects at once. There were several problems with the creation of protoplasts required for the transformations. At the same time we were struggling with the creation of the knockout. We used a method which involved extensive use of PCRs. It took us several weeks instead of several days to get our PCR working, which delayed the creation of our knockout at a crucial moment. However, all these problems combined were not enough to convince our team to stop working on the fungal project. |
For our ambitious project we decided to implement a synthetic construct in <span style="font-style:italic;">A. nidulans</span>. The decision was made to synthesize this construct at a foreign company, in the hope of saving ourselves time. The constructs were made in silico and after certain small corrections the sequences were submitted to the company. The constructs were submitted on time, but soon enough the DNA synthesis company let us know they were struggling with putting the final construct together. We received our first part in time and succesfully transformed <span style="font-style:italic;">A. nidulans</span> and confirmed that our first construct worked. | For our ambitious project we decided to implement a synthetic construct in <span style="font-style:italic;">A. nidulans</span>. The decision was made to synthesize this construct at a foreign company, in the hope of saving ourselves time. The constructs were made in silico and after certain small corrections the sequences were submitted to the company. The constructs were submitted on time, but soon enough the DNA synthesis company let us know they were struggling with putting the final construct together. We received our first part in time and succesfully transformed <span style="font-style:italic;">A. nidulans</span> and confirmed that our first construct worked. |
Revision as of 16:54, 8 September 2011