Team:EPF-Lausanne/Notebook/September2011

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(Thursday, 8 September 2011)
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Nadine made liquid cultures of J61002 Ptet-RFP because we were running out of DNA. We want to send it for sequencing, to be sure that in this plasmid Ptet is OK - although the experiments suggest that yes. She also made liquid cultures of the successful ligations (see yesterday's results) in order to sequence-verify them afterwards.
Nadine made liquid cultures of J61002 Ptet-RFP because we were running out of DNA. We want to send it for sequencing, to be sure that in this plasmid Ptet is OK - although the experiments suggest that yes. She also made liquid cultures of the successful ligations (see yesterday's results) in order to sequence-verify them afterwards.
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== Friday, 09 september 2011 ==
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Nadine's platereader experiment gave disappointing results: the cells didn't grow much, reaching only 0.1-0.2 OD instead of 1 normally. This will be repeated next Tuesday.
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The minipreps of J6 Ptet-RFP and the T7 biobricks yielded high concentrations, and therefore were sent for sequencing.
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{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}

Revision as of 09:10, 9 September 2011