From 2011.igem.org
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| | ==Notebook== | | ==Notebook== |
| - | ===2011-08-05=== | + | ===Week 1=== |
| - | We found the plasmids in the second generation bl21 in wrong sizes after long time reservation in -20℃.
| + | '''Aim:''' |
| - | Our team leader went crazy.
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| | | | |
| - | ===2011-09-02===
| + | By replacing the promoter of the existing BioBrick BBa_F2621 with Placo-1, the expression of the downstream sequence can be controlled by adding IPTG. |
| - | The curve diagram of quantity and that of fluorescence seemed strange. We were confused and frustrated. Through discussions and seeking others for help, we sorted out some reasons that might lead to the failure. It was planned that the experiment would be done another time next week. Methods of dealing with samples will be changed and details in every step will be attached importance to. We expect a good result of the coming week. Cheer up!
| + | '''Performance:''' |
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| - | ===2011-09-05===
| + | Constructing the new BioBrick BBa_K658000 (The figure below and sequence analysis can indicate it is correctly constructed.) |
| - | We added our first biobrick part:BBa_K658000 in the registry.What a nice day!
| + | Testing the expression of BBa_K658000 by adding IPTG |
| - | | + | (We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! ) |
| - | ===2011-09-07===
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| - | We made up the solid culture media in preparation for getting the growth curve of BL21 with the population-control device and BL21 bacteria as a control group. | + | |
| - | When the OD of the inoculated bacteria reached the expected value,the first sampling was made.The conical flask with the bacteria was put back in the bacterial culture shaker for growth.It was planned to take samples every hour in the first 8 hours.
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Revision as of 19:09, 3 October 2011
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