"
Team:Osaka/week6
From 2011.igem.org
(Difference between revisions)
| Line 57: | Line 57: | ||
# Restriction digests of miniprepped parts | # Restriction digests of miniprepped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
| - | # 03 ok | + | #* 03 ok |
# To liquid culture:03,04 (preincubation) | # To liquid culture:03,04 (preincubation) | ||
Revision as of 13:13, 7 September 2011
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
- Absorbance measurement
| Name | IPTG(0.1M) | incubation | OD600 | A474 |
|---|---|---|---|---|
| 1 | - | - | 0.8583 | 0.1567 |
| 2 | 400μl | 2h | 1.1523 | 0.1447 |
| 3 | 400μl | 1h | 0.5583 | 0.1126 |
| 4 | 40μl | 2h | 1.0297 | 0.1878 |
| 5 | 40μl | 1h | 0.6576 | 0.1736 |
- To liquid culture:03,17,3-10C
September 6(Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- all parts ×
- PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
- Gel Electrophoresis of PCR products
- PprM ok
- PprI,PprA,RecA ×
- PCR
- Gel Electrophoresis of PCR products
- ×
September 7(Wed)
- PCR
- Gel electrophoresis of PCR products
- PprM ok
- PprI,PprA,RecA ×
- Gel electrophoresis of PprM
- Gel extraction
- PCR
- Gel electrophoresis of PCR products
- PprI,PprA,RecA ×
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Gel electrophoresis of digests
- 03 ok
- To liquid culture:03,04 (preincubation)
