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Team:WarrenCIndpls IN-HS/Notebook
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==May 19th: Research on Parts== | ==May 19th: Research on Parts== | ||
| + | Kozak Sequence- directs translation of mRNA for more efficiency and accuracy | ||
| + | Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors | ||
| + | Terminator Sequence- signals the end of transcriptoin to RNA polymerase | ||
| + | Vector- Plasmid | ||
| + | ORI (Origin of Replication) | ||
| + | Selection Markers | ||
| + | -Ura3 is a selection marker for yeast | ||
| + | -Ampicillin is a selection marker for bacteria | ||
==May 26th: Primer Design== | ==May 26th: Primer Design== | ||
Revision as of 17:13, 7 June 2011
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Contents |
Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors Terminator Sequence- signals the end of transcriptoin to RNA polymerase Vector- Plasmid
ORI (Origin of Replication)
Selection Markers
-Ura3 is a selection marker for yeast
-Ampicillin is a selection marker for bacteria
