"
26 (May 26, 2011 GDS)
From 2011.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
:-Spin 2.5 min. | :-Spin 2.5 min. | ||
:-Transfer supernatant to clean tube and add 1mL of EtOH | :-Transfer supernatant to clean tube and add 1mL of EtOH | ||
| - | :- | + | :-Mix and pellet DNA by centrifugation for 2-5 min |
| + | :-Wash pellet with 70% ethanol and allow pellet to dry | ||
| + | :-Resuspend the pellet in 30λ of the TE | ||
| + | :-Digest 5-10λ | ||
Revision as of 20:50, 6 September 2011
Created TENS
- 4.5 mL TE and
- 250uL 10% SDS
- 250uL 2N NaOH
- -Spin 4 tubes (1.5mL) 30"
- -Pour off sol'n & resuspend
- -Add
- 300uL TENS: mix
- 150uL NaOH: mix
- -Spin 2.5 min.
- -Transfer supernatant to clean tube and add 1mL of EtOH
- -Mix and pellet DNA by centrifugation for 2-5 min
- -Wash pellet with 70% ethanol and allow pellet to dry
- -Resuspend the pellet in 30λ of the TE
- -Digest 5-10λ
