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26 (May 26, 2011 GDS)
From 2011.igem.org
(Difference between revisions)
m (spelling) |
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| Line 10: | Line 10: | ||
:-Add | :-Add | ||
::300uL TENS: mix | ::300uL TENS: mix | ||
| - | ::150uL | + | ::150uL NaOH: mix |
:-Spin 2.5' | :-Spin 2.5' | ||
:-Transfer supernatant to clean tube and add 1mL of EtOH | :-Transfer supernatant to clean tube and add 1mL of EtOH | ||
:-Vortex and pellet DNA by centrifugation for 2.5mL | :-Vortex and pellet DNA by centrifugation for 2.5mL | ||
Revision as of 20:44, 6 September 2011
Created TENS
- 4.5 mL TE and
- 250uL 10% SDS
- 250uL 2N NaOH
- -Spin 4 tubes (1.5mL) 30"
- -Pour off sol'n & resuspend
- -Add
- 300uL TENS: mix
- 150uL NaOH: mix
- -Spin 2.5'
- -Transfer supernatant to clean tube and add 1mL of EtOH
- -Vortex and pellet DNA by centrifugation for 2.5mL
