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26 (May 26, 2011 GDS)
From 2011.igem.org
(Difference between revisions)
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Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes, test at 1.0 K. Transfer the supernatant to clean tube and ___ 2 volumes (1mL) of room temp ETOH. Vortex and pellet DNA by centrifugation for 2-5 min. at 10 K. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE with RNAseA. Digest 5-10uL as uaual. | Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes, test at 1.0 K. Transfer the supernatant to clean tube and ___ 2 volumes (1mL) of room temp ETOH. Vortex and pellet DNA by centrifugation for 2-5 min. at 10 K. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE with RNAseA. Digest 5-10uL as uaual. | ||
:PNICE--5/12/11 | :PNICE--5/12/11 | ||
| - | :PNICE (tKAN--5/23/11 | + | :PNICE (tKAN)--5/23/11 |
:J04430--5/23/11 | :J04430--5/23/11 | ||
:J04430--5/12/11 | :J04430--5/12/11 | ||
Revision as of 19:16, 5 September 2011
Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing. Add 300uL of TENS and mix by inversion . The solution should become viscous add 150uL of sodium acetate and vortex. A fine white precipitate should form. Centrifuge for 2.5 minutes, test at 1.0 K. Transfer the supernatant to clean tube and ___ 2 volumes (1mL) of room temp ETOH. Vortex and pellet DNA by centrifugation for 2-5 min. at 10 K. Wash pellet with 70% ethanol and allow pellet to dry. Resuspend the pellet in 30uL TE with RNAseA. Digest 5-10uL as uaual.
- PNICE--5/12/11
- PNICE (tKAN)--5/23/11
- J04430--5/23/11
- J04430--5/12/11
- Trans 003-W1--5/12/11
- Trans 003-W1--5/23/11
P.P. G.E. S.D.
