Team:Osaka/week6
From 2011.igem.org
(Difference between revisions)
Line 15: | Line 15: | ||
!Name!!IPTG(0.1M)!!incubation!!OD600!!A474 | !Name!!IPTG(0.1M)!!incubation!!OD600!!A474 | ||
|- | |- | ||
- | |1- | + | |1||-||2h||0.8583||0.1567 |
|- | |- | ||
- | | | + | |2||400μl||2h||1.1523||0.1567 |
|- | |- | ||
- | | | + | |3||400μl||1h||0.5583||0.1567 |
|- | |- | ||
- | | | + | |4||40μl||2h||1.0297||0.1567 |
|- | |- | ||
- | | | + | |5||40μl||1h||0.6576||0.1567 |
|} | |} | ||
Revision as of 12:33, 5 September 2011
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
Name | IPTG(0.1M) | incubation | OD600 | A474 |
---|---|---|---|---|
1 | - | 2h | 0.8583 | 0.1567 |
2 | 400μl | 2h | 1.1523 | 0.1567 |
3 | 400μl | 1h | 0.5583 | 0.1567 |
4 | 40μl | 2h | 1.0297 | 0.1567 |
5 | 40μl | 1h | 0.6576 | 0.1567 |