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Team:Freiburg/Notebook/3 September
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[[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]] | [[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]] | ||
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| + | the samples 46b= ho1 with a RBS (BBa_B0034)<br/> | ||
| + | 50b= RBS(BBa_B0034)+pcyA+terminator<br/>, | ||
| + | 64b= Promotor (BBa_J23110)+CcaS<br/> | ||
| + | 67b= romotor (BBa_J23110)+CcaR<br/> | ||
| + | were send to GATC for sequencing | ||
===3A-assembly:Digestion=== | ===3A-assembly:Digestion=== | ||
Revision as of 11:06, 21 September 2011
Contact 
Contents |
green light receptor
Testdigestion of 2a-assembly
Investigators: Julia
Continuing from 31 of Aug. [1]
Before Miniprep of 20 different colonies.
Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.
Result:
the samples 46b= ho1 with a RBS (BBa_B0034)
50b= RBS(BBa_B0034)+pcyA+terminator
,
64b= Promotor (BBa_J23110)+CcaS
67b= romotor (BBa_J23110)+CcaR
were send to GATC for sequencing
3A-assembly:Digestion
Investigators: Sandra
Digestion of:
InsertI:
- 64
- 46
with EcorI and SpeI
InsertII:
- 67
- 50
with XbaI and PstI
Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour.
3A-assembly:Ligation
Investigators: Julia
blue light receptor
Stop of ligation
Investigators: Sandra
Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.
To do: Trafo
Transformation
with ♥C3 Lig. and Not C3 Lig
Investigator: Sophie
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Picking clones
Investigators: Sandra
Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.
