Team:Warsaw/Safety

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       <ul>
       <ul>
         <li> researcher safety, </li>
         <li> researcher safety, </li>
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Reasearchers safety:<br>
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Work in a microbiology lab enviroment:<br>
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Work in a microbiology lab environment:<br>
In the lab there is work with GMO and E.Coli bacteria strains
In the lab there is work with GMO and E.Coli bacteria strains
Researchers have to wear lab coats and gloves
Researchers have to wear lab coats and gloves
Work with toxic chemical compounds:<br>
Work with toxic chemical compounds:<br>
Ethidium Bromide can cause cancer -  is used only to visualize gels
Ethidium Bromide can cause cancer -  is used only to visualize gels
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It is not added to agarose gels before running the gel.
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It is not added to agarose gels before running the gel.<br>
Antibiotics: Chloramphenicol and Ampicillin:<br>
Antibiotics: Chloramphenicol and Ampicillin:<br>
While making antibiotics solutions from the powder the mask
While making antibiotics solutions from the powder the mask
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and safety glasses have to be worn
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and safety glasses have to be worn<br>
Work in the RNAse free environment:<br>
Work in the RNAse free environment:<br>
Hazards include RNAse cleaners like RNA zap, a mask should be worn.
Hazards include RNAse cleaners like RNA zap, a mask should be worn.
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The cleaners are irritating
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The cleaners are irritating<br>
Work with UV light:<br>
Work with UV light:<br>
UV light is a cause of cancer therefore a UV protective mask, gloves
UV light is a cause of cancer therefore a UV protective mask, gloves
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Our department also obtained the necessary permission to work with GMO organisms. Therefor it is fully legal to engineer E. Coli according to Polish law<br>  
Our department also obtained the necessary permission to work with GMO organisms. Therefor it is fully legal to engineer E. Coli according to Polish law<br>  
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     </li>
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   <li> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </li>
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<Strong>
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   <li> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </li></strong>
We designed a protocol to perform cloning without use of living organisms
We designed a protocol to perform cloning without use of living organisms
This protocol allows cloning biobricks without the need to produce GMO organisms.
This protocol allows cloning biobricks without the need to produce GMO organisms.

Revision as of 15:10, 3 September 2011


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Safety

  1. Would any of your project ideas raise safety issues in terms of:
    • researcher safety,
      • Work in a microbiology lab environment:
      In the lab there is work with GMO and E.Coli bacteria strains Researchers have to wear lab coats and gloves Work with toxic chemical compounds:
      Ethidium Bromide can cause cancer - is used only to visualize gels It is not added to agarose gels before running the gel.
      Antibiotics: Chloramphenicol and Ampicillin:
      While making antibiotics solutions from the powder the mask and safety glasses have to be worn
      Work in the RNAse free environment:
      Hazards include RNAse cleaners like RNA zap, a mask should be worn. The cleaners are irritating
      Work with UV light:
      UV light is a cause of cancer therefore a UV protective mask, gloves and lab coats have to be worn while working with the UV light
    • public safety, or environmental safety?
      • The microbiological wastes should be properly disposed - as adviced by the university to ensure that no GMO organisms or toxic compounds gets to the environment The used agarose gels have to be properly disposed.
    • Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
       No, currently our planned BioBricks are completly biosafe. We use only construct expressing fluorescent proteins. The new brick inclused modified RBS parts To our best knowledge those bricks are safe to use in the lab
    • Is there a local biosafety group, committee, or review board at your institution?
      In Poland there are no biosafety groups locally at the universities. We are following Biosafety guidelines of Polish Ministry of Environment. Our department also obtained the necessary permission to work with GMO organisms. Therefor it is fully legal to engineer E. Coli according to Polish law
    • Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
      • We designed a protocol to perform cloning without use of living organisms This protocol allows cloning biobricks without the need to produce GMO organisms. Therefor according to Polish law and biosafety rules it allows even high schools to take part in iGEM The Genetically Modified Organism is create only at final stage - to test the device. It can be performed at GMO certified institution

 

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