Team:Tsinghua/Notebook/4 June 2011

From 2011.igem.org

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Line 6: Line 6:
   gel extraction.
   gel extraction.
   check the concentration of PW solution each time
   check the concentration of PW solution each time
-
      blank DELL 2011-6-4 17:02:14 0.6 ng/µl
+
 
-
final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl
+
blank DELL 2011-6-4 17:02:14 0.6 ng/µl
-
2nd PW DELL 2011-6-4 17:03:39 2.4 ng/µl
+
 
-
3rd PW DELL 2011-6-4 17:04:10 1.5 ng/µl
+
final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl
 +
 
 +
2nd PW DELL 2011-6-4 17:03:39 2.4 ng/µl
 +
 
 +
3rd PW DELL 2011-6-4 17:04:10 1.5 ng/µl
 +
 
   still get low concentration!!!
   still get low concentration!!!

Latest revision as of 14:40, 2 September 2011

pet15b

  digest and mini pre.
  I2011-06-04 pet15b BamHI+NdeI digestion.jpg
  gel extraction.
  check the concentration of PW solution each time

blank DELL 2011-6-4 17:02:14 0.6 ng/µl

final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl

2nd PW DELL 2011-6-4 17:03:39 2.4 ng/µl

3rd PW DELL 2011-6-4 17:04:10 1.5 ng/µl

  still get low concentration!!!