Team:Freiburg/Notebook/19 September

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/18_September">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 19 September </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/20_September">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===The_little_hoped_for_experiment_IV===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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I took the 3 cultures and prepared some tubes for them to test... I made measurements of the OD after about 18h of incubation at 30°C, put 1ml from every culture in an eppi (2 eppis for every culture) and incubated 3 of them at 30°C and 3 at 42°C.
 +
The results can be seen on our Project Results page or the Registry (Part BBa_K608351 and BBa_K608352).
 +
 +
After that, I sent the samples for sequencing hoping I would get the results the next day, but typical GATC decided to delay them until the 21st of September!!!
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
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===Ligation===
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name:Rüdiger
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date:19.08.
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Date Name
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Experiment Digestion19.08. Rüdiger /Gelextraction (Theo)
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name:Precipitator
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|}
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'''Procedure'''
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PCR tube:
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total volume 20 μl
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# add H<sub>2</sub>O (17 μl -X-Y-Z)
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# add 2 μl Ligase Buffer 10x
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# add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
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# add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
 +
# Add 1 μl T4-DNA Ligase
 +
# Incubate 10-30 min at room temperature
 +
# heat for 20 minutes at 80°C
 +
# store at -20°C or directly proceed to transformation
 +
 +
 +
{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name of part
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Ratio Insert:Vector
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<nowiki>= 3:1 or 1:1</nowiki>
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Volume (μl)
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| X insert 1
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| <center>01:03</center>
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 10
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Y insert 2
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Z vector
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2
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|-
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| H<sub>2</sub>O
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| style="background-color:#bfbfbf;border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="background-color:#bfbfbf;border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"|
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 5
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|}
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'''Documentation:'''
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 +
Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.
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 +
 +
{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Trying to ligate the 3 Precipitator motifs into iGEM Vector pSB1C3
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|}
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===NAME OF YOUR EXPERIMENT===
===NAME OF YOUR EXPERIMENT===

Latest revision as of 00:21, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

The_little_hoped_for_experiment_IV

Investigators:Theo


I took the 3 cultures and prepared some tubes for them to test... I made measurements of the OD after about 18h of incubation at 30°C, put 1ml from every culture in an eppi (2 eppis for every culture) and incubated 3 of them at 30°C and 3 at 42°C. The results can be seen on our Project Results page or the Registry (Part BBa_K608351 and BBa_K608352).

After that, I sent the samples for sequencing hoping I would get the results the next day, but typical GATC decided to delay them until the 21st of September!!!

Precipitator

Ligation

Name:Rüdiger Date:19.08.
Continue from Date Name

Experiment Digestion19.08. Rüdiger /Gelextraction (Theo)

Project Name:Precipitator

Procedure


PCR tube:

total volume 20 μl


  1. add H2O (17 μl -X-Y-Z)
  2. add 2 μl Ligase Buffer 10x
  3. add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
  4. add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
  5. Add 1 μl T4-DNA Ligase
  6. Incubate 10-30 min at room temperature
  7. heat for 20 minutes at 80°C
  8. store at -20°C or directly proceed to transformation


Name of part Ratio Insert:Vector

= 3:1 or 1:1

Volume (μl)
X insert 1
01:03
 10
Y insert 2
Z vector 2 2
H2O 5

Documentation:

Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.


Trying to ligate the 3 Precipitator motifs into iGEM Vector pSB1C3


NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/19_September"