Team:Wisconsin-Madison

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<li><a href="#" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
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<a href="#">Main</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
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<a href="#">What is iGEM?</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ca">Contributions & Attributions</a>
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<li><a href="#" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
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<a href="#">Overview</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
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<a href="#">Tiger Blood Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
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<a href="#">Bacardi 151 Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
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<a href="#">Hidden Sleeve Compartment</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/parts">Parts</a>
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<a href="#">Overview</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
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<a href="#">Members</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
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<a href="#">Faculty</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
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<a href="#">Protocols</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calendar</a>
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<a href="#">Experiment</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
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<a href="#">References</a>
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<a href="#">Science Olympiad</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/scienceolympiad">Science Olympiad</a>
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<a href="#">Lectures</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
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<a href="#">Science Olympiad</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/humanpractice">Human Practice</a>
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<a href="#">Lectures</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/team">Link text</a>
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<p><strong><a href="https://2011.igem.org/Main_Page">iGEM 2011</a>: Wisconsin-Madison</strong><p>
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Main Project Title
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This summer, the UW-Madison <a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">iGEM</a> team will be working on streamlining the <a href="https://2011.igem.org/Team:Wisconsin-Madison/biofuel">biofuel</a> discovery and production processes through the use of <a href="https://2011.igem.org/Team:Wisconsin-Madison/biosensor">biosensors</a>. The necessity for sustainable, economical sources of fuel is ever growing, and <a href="http://www.wisc.edu/">UW-Madison</a> is a forefront institution in the hunt for such supplies. In association with the Great Lakes Bioenergy Research Center (<a href="http://www.glbrc.org/">GLBRC</a>), we are creating new E. coli biosensors that can accelerate high throughput screening of potential fuel sources. We’re specifically interested in <a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">ethanol</a> and <a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">alkane</a>, derived from sources ranging from cellulose to metabolically engineered E. coli.  
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus venenatis congue nulla nec tincidunt. Praesent porta vehicula magna eget accumsan. Morbi vel dolor urna. Nunc ullamcorper nisi sit amet enim faucibus eu laoreet arcu lacinia. Vivamus elit felis, tristique vitae eleifend nec, pretium ac tortor. Suspendisse potenti. Curabitur at eros purus, ac rhoncus velit. Morbi luctus sagittis accumsan. Praesent id odio est. Nulla a consequat mi. Aenean tincidunt neque consectetur est commodo at consequat metus condimentum. In porttitor diam id tortor facilisis facilisis. In condimentum turpis nec nisi dictum interdum. Suspendisse est lorem, semper sed tempus ac, faucibus a orci. Pellentesque eleifend dui quis mauris luctus pretium.
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What is iGem?
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iGEM stands for Internationally Gentically Engineered Machines. It began as a friendly competition between 10 MIT teams in 2004, and has since grown to over 130 teams in 2010 from all different countries around the globe. This year even more teams are anticipated, and there will be regional competitions held before the annual international jamboree held at MIT in the fall.
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<p>
<p>
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iGem revolves around two main ideas: Biobricks and the Registry. Biobricks are pieces of DNA that have been given a similar structure and that code for something useful. They can be big or small and may contain one or many individual pieces of useful information all packaged into one coherent piece. The “packaging” for each Biobrick is the same, facilitating the use of the parts.
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We have found regulatory systems which respond to each of the biofuels of interest, and are using standard <a href="https://2011.igem.org/Team:Wisconsin-Madison/biobrick">BioBricks</a> assembly to create E. coli strains which can be used to perform <a href="https://2011.igem.org/Team:Wisconsin-Madison/platereader"> fluorescence-based</a> assays. By using fluorescent biosensors, we hope to lower costs (in both equipment and cost-per-sample) while maintaining a high degree of accuracy. In the interest of creating robust and accurate assays, we are also attempting to increase the magnitude and range of the linear fluorescence response through directed evolution. We hope to leverage multiple selections to both decrease basal fluorescence and increase the point where the response becomes saturated.  
<p>
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Each new part, once confirmed, is sent to MIT and physically stored there in the Registry. Any other iGem team can order any Biobrick from the Rigistry for use in their project, and get physical copies that are easily cultured and can be quickly used, without having to be manufactured by the teams. This open source structure speeds up progress and makes it easier for researchers to accomplish their projects.  
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As a more direct approach to increasing microbial biofuel yields, we also pursued the use of <a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">bacterial microcompartments</a> (BMCs) as scaffolding for key enzymes in the ethanol producing and sensing processes. Through localizing crucial anabolic enzymes, as well as the beginning of our sensing cascades, to the BMC surfaces, we can increase fuel titers as well as our reliability in accurately sensing them. However, due to the complexity of this project and the time investment needed, we have stopped work on the BMC. More information can be found on the <a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartments</a> page.
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<p><br>
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We hope that when combined our projects will produce a useful toolkit for researchers in and beyond the GLBRC to aid the discovery and engineering of promising fuel sources and processes.  
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<p><br> <p>
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<b>Click <a href="https://2011.igem.org/Team:Wisconsin-Madison/ca">here</a> to see our contributions and attributions page.</b>
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<p>
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<b>And <a href="https://2011.igem.org/Team:Wisconsin-Madison/data">here</a> to see our data page.</b>
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Latest revision as of 23:14, 28 September 2011









iGEM 2011: Wisconsin-Madison

This summer, the UW-Madison iGEM team will be working on streamlining the biofuel discovery and production processes through the use of biosensors. The necessity for sustainable, economical sources of fuel is ever growing, and UW-Madison is a forefront institution in the hunt for such supplies. In association with the Great Lakes Bioenergy Research Center (GLBRC), we are creating new E. coli biosensors that can accelerate high throughput screening of potential fuel sources. We’re specifically interested in ethanol and alkane, derived from sources ranging from cellulose to metabolically engineered E. coli.

We have found regulatory systems which respond to each of the biofuels of interest, and are using standard BioBricks assembly to create E. coli strains which can be used to perform fluorescence-based assays. By using fluorescent biosensors, we hope to lower costs (in both equipment and cost-per-sample) while maintaining a high degree of accuracy. In the interest of creating robust and accurate assays, we are also attempting to increase the magnitude and range of the linear fluorescence response through directed evolution. We hope to leverage multiple selections to both decrease basal fluorescence and increase the point where the response becomes saturated.

As a more direct approach to increasing microbial biofuel yields, we also pursued the use of bacterial microcompartments (BMCs) as scaffolding for key enzymes in the ethanol producing and sensing processes. Through localizing crucial anabolic enzymes, as well as the beginning of our sensing cascades, to the BMC surfaces, we can increase fuel titers as well as our reliability in accurately sensing them. However, due to the complexity of this project and the time investment needed, we have stopped work on the BMC. More information can be found on the Microcompartments page.


We hope that when combined our projects will produce a useful toolkit for researchers in and beyond the GLBRC to aid the discovery and engineering of promising fuel sources and processes.


Click here to see our contributions and attributions page.

And here to see our data page.



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