Team:Freiburg

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Latest revision as of 21:42, 21 September 2011

Contact

This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Until the Wikifreeze:

Welcome to the FreiGEM2011 Team Page!

Team

Meet the team of young and enthusiasthic Freiburger scientists: FreiGEM 2011

Project

We aim to transform protein expression in bacterial systems into an elegant, fast and affordable process. By eliminating routine use of expensive materials, this novel assay will utilize sustainable laboratory equipment and widespread His-Tag technology.

SynBio Oath

As part of our considerations in biosafety and security we found that good conduct in research and development is a matter of personal motivation. This was incentive for us to propose something akin to the Hippocratic Oath for Biologists.

Lab in a cell video

Turn on english subtitles by clicking on the "cc" button in the player.

What can the Lab in a Cell do?

We are creating a genetically engineered organism that will work as a tool for the expression of any desired His-tagged protein, plus a lysis casette and a precipitator that binds to his-tags on one end and the plastic of pipette-tips on the other, making washing and purification a simple process with no need for either centrifuges or purification columns. If successful, this will drastically improve the on-site impact of synthetic biology, taking the lab to where it is needed - even if that is a long way away from high-tech facilities. In order to get the desired protein, all that is needed will be a plasmid encoding the wanted protein with an added his-tag and the light-sensitive promoter in front which can be specifically induced by our light switch. If the principle proves sound, it may even be introduced to yeast cultures, enabling the researcher to take the needed cultures with him in lyophilized form, making even refrigerators unnecessary – just add water + sugar and the needed proteins can be expressed and purified by means of a few handheld devices such as a thermometer, an LED-set with the specified wavelengths and pipettes. Imagine being able to get all the enzymes necessary for cloning from powders, requiring no more than a dry place for storage and a few simple devices and ingredients for the activation of our tool:

- an LED device able to give off six specific wavelengths

- an incubator (although the right choice of host-organisms may also enable a field researcher to use his own body temperature)

- a pipette and a couple of tips

- some wash- and elution buffer

- plastic tubes

Although not a stand-alone creation, we believe we can give a nifty little piece of equipment to the community of synthetic biologists and pharmacists, saving time and effort for small-scale experimentation and on-site research as well as facilitating low budget pharmaceutical research and production, a boon to places where access to equipment and consumables is limited.

Visits on our wiki

Biology is like sex: you don't do it for the outcome...

@@ Line 79: / Line 79: @@
 	 	<div id=freiburg_box style="float:left">
 	  		<div id = freiburg_box_content>
-				<h2>Work highlights</h2>
+				<h2>SynBio Oath</h2>
-				<img src="https://static.igem.org/mediawiki/2011/9/91/Work_in_progress.png" width = "180px"/>
+				<p>As part of our considerations in biosafety and security we found that good conduct in research and development is a matter of personal motivation. This was incentive for us to propose something akin to the Hippocratic Oath for Biologists. </p>
 	  		</div>
+			<a href="https://2011.igem.org/Team:Freiburg/AspirationalCode" class="more" title="Continue reading about our Project">Read more<span class="blue"> > </span></a>
 	 	</div>
@@ Line 106: / Line 107: @@
 <br/>
-===The Code===
+==  What can the Lab in a Cell do?  ==
+We are creating a genetically engineered organism that will work as a tool for the expression of any desired His-tagged protein, plus a lysis casette and a precipitator that binds to his-tags on one end and the plastic of pipette-tips on the other, making washing and purification a simple process with no need for either centrifuges or purification columns.  If successful, this will drastically improve the on-site impact of synthetic biology, taking the lab to where it is needed - even if that is a long way away from high-tech facilities. In order to get the desired protein, all that is needed will be a plasmid encoding the wanted protein with an added his-tag and the light-sensitive promoter in front which can be specifically induced by our light switch. If the principle proves sound, it may even be introduced to yeast cultures, enabling the researcher to take the needed cultures with him in lyophilized form, making even refrigerators unnecessary – just add water + sugar and the needed proteins can be expressed and purified by means of a few handheld devices such as a thermometer, an LED-set with the specified wavelengths and pipettes. Imagine being able to get all the enzymes necessary for cloning from powders, requiring no more than a dry place for storage and a few simple devices and ingredients for the activation of our tool:
-|As mentioned at the Meeting in Carmona we are developing an ethical code for biologists:<br />
+-	an LED device able to give off six specific wavelengths
-Here we present you the nearly finished aspirational code developed at the DIYbio European Congress in London, on the 8th of May. <br />
+-	an incubator (although the right choice of host-organisms may also enable a field researcher to use his own body temperature)
-*Know you don’t know everything
-*Recognize the complexity and dynamics of living systems and our responsibility towards them
-*Respect humans and all living systems
-*Listen and respond to concerns
-*Adopt safe practices
-*Enjoy your work
-*Strive to increase public understanding of the methods, results and implications of the 21st century biology
-*Emphasize the sharing of ideas, knowledge, data and results
-This  code draft was created by several young scientists and amateur biology enthusiasts. We all had the feeling that public perception of our intentions and actions are distorted and that these need to be clarified.For example, many journalists linked us in top articles with bioterrorism which is completely wrong and neither helps us or the public opinion about science in general.<br />
-We want to present ourselves to the public the way we are and hope that this honest and upright approach can change opinions. Furthermore, we plan to create an active, free and open international community for biologists and enthusiasts to exchange ideas, travel around to learn from others and to help and teach each other. For this community we need a recognizable and distinct appearance. We have considered a code, or an oath which we would voluntarily make, clearly telling everyone who we are, what we want and do not want and what we are doing. This will provide the appearance to increase the value and reputation of the biologist in general.<br />
-The Code above is simply a starting point to find a common ground. It needs to be elaborated upon and there will probably be several sub codes of conduct in the future,which will be adapted to local needs and laws.<br />
-iGEM would be a good forum to promote the idea among scientists and public and build a long lasting community. It has to be emphasized here, that this code should become universal for all biologists, not only restricted to iGEM or the DIYbio community. This will guarantee longevity and a bigger impact of the code and the community in future.
-<br />
-<br />
-If you are interested in joining the effort to further develop this important code, please  [mailto:freigem2011@googlemail.com contact] us!
-|}
-<html >
+-	a pipette and a couple of tips
+-	some wash- and elution buffer
+-	plastic tubes
+Although not a stand-alone creation, we believe we can give a nifty little piece of equipment to the community of synthetic biologists and pharmacists, saving time and effort for small-scale experimentation and on-site research as well as facilitating low budget pharmaceutical research and production, a boon to places where access to equipment and consumables is limited.
+<html>
 <div id=visitorsMap  align=center>
-<img style="visibility:hidden;width:0px;height:0px;" border=0 width=0 height=0 src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMDc*NTYwNzU1MjYmcHQ9MTMwNzQ1NjA4NzE5NiZwPTYzNTAzMiZkPSZnPTEmbz*1YjI2NDg3N2FlOTQ*ODU5OGQ3/NGRmYjY4OGI*NWNkMSZvZj*w.gif" />                <a href="http://www.ipligence.com/visitor_extended/31f26d3bee26ac0656114936ee806002/year"><img src="http://www.ipligence.com/visitor-maps/m/small/1/year/31f26d3bee26ac0656114936ee806002" alt="Visit http://www.ipligence.com" style="border:0;" /></a>
+<img style="visibility:hidden;width:0px;height:0px;" border=0 width=0 height=0 src="http://c.gigcount.com/wildfire/IMP/CXNID=2000002.0NXC/bT*xJmx*PTEzMDc*NTYwNzU1MjYmcHQ9MTMwNzQ1NjA4NzE5NiZwPTYzNTAzMiZkPSZnPTEmbz*1YjI2NDg3N2FlOTQ*ODU5OGQ3/NGRmYjY4OGI*NWNkMSZvZj*w.gif" />
-<table width="133" border="0" cellspacing="0" cellpadding="3"><tr><td align="center"><a href="size=25" target="_blank"><img src="http://www.website-hit-counters.com/cgi-bin/image.pl?URL=616548-7105" alt="size=25" title="size=25" border="0" ></a></td></tr><tr><td align="center"><font style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #330006; text-decoration: none;"> <a href="http://www.sportsnews.be/" target="_blank" style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #555556; text-decoration: none;" title="Visits on our wiki">Visits on our wiki</a> </font></td></tr></table>
+<a href="http://www.ipligence.com/visitor_extended/31f26d3bee26ac0656114936ee806002/year"><img src="http://www.ipligence.com/visitor-maps/m/small/1/year/31f26d3bee26ac0656114936ee806002" alt="Visit http://www.ipligence.com" style="border:0;" /></a>  <BR><BR>
-</div>
+<table align="center" width="133" border="0" cellspacing="0" cellpadding="3"><tr><td align="center"><a href="size=25" target="_blank"><img src="http://www.website-hit-counters.com/cgi-bin/image.pl?URL=616548-7105" alt="size=25" title="size=25" border="0" ></a></td></tr><tr><td align="center"><font style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #330006; text-decoration: none;"> <a href="http://www.sportsnews.be/" target="_blank" style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #555556; text-decoration: none;" title="Visits on our wiki">Visits on our wiki</a> </font></td></tr></table>
+<marquee> <font size="-4">Biology is like sex: you don't do it for the outcome...</font></marquee>
 </html>
-{{:Team:Freiburg/Templates/footer}}
-----
+{{:Team:Freiburg/Templates/footer}}
-references:<br/>
-Tabor, J. J., Levskaya, A., & Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. Journal of Molecular Biology, 405(2), 315-324. Elsevier Ltd. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/21035461