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Team:Michigan/Protocols
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=Protocols= | =Protocols= | ||
| - | '''Basic protocols''' ::: These protocols are necessary for all lab operations | + | '''Basic protocols''' ::: These protocols are necessary for all lab operations. |
[[Media: Autoclaving100.pdf | Autoclave usage]] | [[Media: Autoclaving100.pdf | Autoclave usage]] | ||
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[[Media: OD600_100.pdf | OD600 optimization]] | [[Media: OD600_100.pdf | OD600 optimization]] | ||
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| + | '''Gels and electrophoresis''' ::: These protocols contain information on the production and usage of gels. | ||
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| + | [[Media: Electrophoresis102.pdf | Gel electrophoresis]] | ||
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| + | [[Media: Gel_viewer.pdf | Using the gel viewer]] | ||
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[[Media: electroporate.pdf | Electroporation of electrocompetent cells]] | [[Media: electroporate.pdf | Electroporation of electrocompetent cells]] | ||
| - | [[Media: competent.pdf | Production of standard competent cells]] | + | [[Media: competent.pdf | Production of standard/QT competent cells]] |
[[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | [[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | ||
| + | [[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]] | ||
'''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | '''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | ||
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| + | [[Media: quick-ligase.pdf | Using quick ligase]] | ||
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| + | [[Media: T4-ligase.pdf | Using T4 ligase]] | ||
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| + | [[Media: digests.pdf | Using Restriction enzymes]] | ||
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| + | '''Biobrick specific''' ::: These protocols contain information specific to the usage of Biobricks. | ||
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| + | [[Media: biobrick_removal.pdf | Extraction of BioBricks from wells in the distribution]] | ||
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Latest revision as of 22:31, 28 September 2011
Protocols
Basic protocols ::: These protocols are necessary for all lab operations.
Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.
Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.
Production of electrocompetent cells
Electroporation of electrocompetent cells
Production of standard/QT competent cells
Quick Transformation (QT) of standard competent cells
Heat shock transformation of standard competent cells.
Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
Biobrick specific ::: These protocols contain information specific to the usage of Biobricks.
