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Team:Caltech/Week 10
From 2011.igem.org
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Plate and sequence 16s vectors and WT-F87A<br/> | Plate and sequence 16s vectors and WT-F87A<br/> | ||
Make glycerol stocks of WT-F87A<br/> | Make glycerol stocks of WT-F87A<br/> | ||
| + | Made new DH5a electrocompetent cells with Nate<br/> | ||
===Results=== | ===Results=== | ||
Electrospray of BPA in MeOH and its degraded products show a mass reduction of 22<br/> | Electrospray of BPA in MeOH and its degraded products show a mass reduction of 22<br/> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
| - | Most of the colonies on the positive plates were blue, indicating the presence of working lacZ. | + | Most of the colonies on the positive plates were blue, indicating the presence of working lacZ.<br/> |
| + | <gallery> | ||
| + | File:R0010lacz.jpg|LacZ with inducible promoter in XL-10 gold cells | ||
| + | File:J23119lacz.jpg|LacZ with constitutive promoter in XL-10 gold cells | ||
| + | </gallery> | ||
==August 20== | ==August 20== | ||
Visit West Basin Municipal Water Facility<br/> | Visit West Basin Municipal Water Facility<br/> | ||
| + | <gallery>File:DSC00440.jpg|Tour is a hard-hat because of the golf ball course next to West Basin | ||
| + | File:DSC00446.jpg|Assistant general manager Shivaji Deshmukh explaining the water treatment process | ||
| + | File:DSC00449.jpg|Walking up to the treatment tanks | ||
| + | File:DSC00450.jpg|Ferric chloride and settling treatment | ||
| + | File:DSC00458.jpg|Green tank: biological treatment; Red tank: ferric chloride | ||
| + | File:DSC00465.jpg|Explaining microfiltration columns using bacteria plushie models | ||
| + | File:DSC00467.jpg|Microfiltration units | ||
| + | File:DSC00471.jpg|Reverse osmosis tanks | ||
| + | File:DSC00474.jpg|Explaining reverse osmosis using more bacteria plushie models | ||
| + | File:DSC00475.jpg|UV light treatment | ||
| + | File:DSC00480.jpg|Taste test of treated water purity | ||
| + | </gallery> | ||
}} | }} | ||
Latest revision as of 23:48, 25 August 2011
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Project |
August 15CPCR of Gibson pNT002 and pNT003 ResultsCPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked. August 16Electrospray bisphenol A dissolved in methanol ResultsElectrospray results show correct mass spectrometry peaks
August 17Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces ResultsThe lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.
August 18Restriction digest of R0010 and J23119 and their backbone for lacZ construct. Ligation of each promoter/backbone with lacZ purified yesterday. Transformed in XL-10 gold Results4 colonies on pSB1C3 plate, all pink. August 19Visit Monk Hill Groundwater Treatment Plant ResultsElectrospray of BPA in MeOH and its degraded products show a mass reduction of 22
Number of colonies on X-gal-ampicillin-LB plates
Most of the colonies on the positive plates were blue, indicating the presence of working lacZ.
August 20Visit West Basin Municipal Water Facility
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