Team:Arizona State/Parts

From 2011.igem.org

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== BBa_K578001 ==
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'''E. coli CRISPR I leader sequence'''
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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This is the AT-rich leader region of the CRISPR I array in Escherichia coli str. K-12 substr. MG1655. "Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS" (Ü. Pul et al, 2010) characterized a single promoter (Pcrispr1) in this sequence. Transcription is silenced through H-NS binding to the leader sequence. Induction requires anti-silencing, either by protein-independent processes, DNA-binding proteins or other modulatory mechanisms not yet identified.
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== BBa_K578002 ==
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'''T7LRSR (Listeria innocua CLIP11262)'''
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[[image: BBa_K578002.png|800px]]
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This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.
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== BBa_K578003 ==
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'''RSR Array Segment (Listeria innocua CLIP11262)'''
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[[image: BBa_K578003.png|800px]]
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{|align="justify"
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This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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== BBa_K578004 ==
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|[[Image:Arizona_State_logo.png|200px|right|frame]]
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'''Bacillus halodurans C-125 CRISPR leader sequence'''
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''Tell us more about your project. Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Arizona_State_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Arizona_State | Team Example]]
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<!--- The Mission, Experiments --->
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This is a putative leader sequence for the CRISPR III locus in Bacillus halodurans C-125.
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== BBa_K578005 ==
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'''J23102+E0840 (Constitutive GFP)'''
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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This is a combination of the GFP coding region (E0840), and the medium strength constitutive promoter, J23102.
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!align="center"|[[Team:Arizona_State|Home]]
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[[image: ASU_BBa_K578005_2.png|800px]]
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!align="center"|[[Team:Arizona_State/Team|Team]]
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<!--<groupparts>iGEM11_Arizona_State</groupparts>-->
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Arizona_State Official Team Profile]
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!align="center"|[[Team:Arizona_State/Project|Project]]
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!align="center"|[[Team:Arizona_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Arizona_State/Modeling|Modeling]]
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!align="center"|[[Team:Arizona_State/Notebook|Notebook]]
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!align="center"|[[Team:Arizona_State/Safety|Safety]]
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!align="center"|[[Team:Arizona_State/Attributions|Attributions]]
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|}
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}} <!-- close content attribute for menubar -->
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===Parts===
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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<groupparts>iGEM010 Arizona_State</groupparts>
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Latest revision as of 03:12, 29 September 2011


Parts


Home iGEM 2011 Home Project
Lab
Results
Notebook
Human Practices

ASU Logo.png
 

Contents

BBa_K578001

E. coli CRISPR I leader sequence

This is the AT-rich leader region of the CRISPR I array in Escherichia coli str. K-12 substr. MG1655. "Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS" (Ü. Pul et al, 2010) characterized a single promoter (Pcrispr1) in this sequence. Transcription is silenced through H-NS binding to the leader sequence. Induction requires anti-silencing, either by protein-independent processes, DNA-binding proteins or other modulatory mechanisms not yet identified.

BBa_K578002

T7LRSR (Listeria innocua CLIP11262) BBa K578002.png

This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.

BBa_K578003

RSR Array Segment (Listeria innocua CLIP11262)

BBa K578003.png

This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.

BBa_K578004

Bacillus halodurans C-125 CRISPR leader sequence

This is a putative leader sequence for the CRISPR III locus in Bacillus halodurans C-125.

BBa_K578005

J23102+E0840 (Constitutive GFP)

This is a combination of the GFP coding region (E0840), and the medium strength constitutive promoter, J23102.

ASU BBa K578005 2.png

Contact Us

Arizona State University

ECG 334, PO BOX 9709

Tempe, Arizona 85287

Email

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