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<h3>FIGURES</h3>
<h3>FIGURES</h3>
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<h2>Figure 1</h2>
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<a name="fig01"><h2>Figure 1</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig01.jpg" border="0"><br><br>
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Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig02"><h2>Figure 2</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig02.jpg" border="0"><br><br>
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Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator.
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig03"><h2>Figure 3</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig03.jpg" border="0"><br><br>
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Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor.
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig04"><h2>Figure 4</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig04.jpg" border="0"><br><br>
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Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, <i>H</i><sub>2</sub>= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 3<sub>1</sub>= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 3<sub>1</sub>= Mnt repressor, <i>H</i><sub>1</sub>= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI.
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig05"><h2>Figure 5</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig05.jpg" border="0"><br><br>
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Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor.
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig06"><h2>Figure 6</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig06.jpg" border="0"><br><br>
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Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig07"><h2>Figure 7</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig07.jpg" border="0"><br><br>
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Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig08"><h2>Figure 8</h2></a><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig08.jpg" border="0"><br><br>
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Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI.
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<a name="fig09"><h2>Figure 9</h2></a><br>
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<img src="http://www.shbts.nl/igem/images/notebook/wetlab_fig01.jpg" border="0"></a><br><br>
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<img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig09.jpg" border="0"><br><br>
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Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L<br><br><a href="javascript: history.go(-1)"><u>Click here to go back</u></a><br><br><br>
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<br><br><a href="javascript: history.go(-1)"><i>&larr; Click here to go back</i></a><br><br><br>
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<h2>Figure 2</h2>
 
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Latest revision as of 11:51, 19 September 2011

KULeuven iGEM 2011

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FIGURES

Figure 1




Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K1= terminator, K2= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L

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Figure 2




Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator.

← Click here to go back


Figure 3




Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor.

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Figure 4




Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, H2= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 31= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 31= Mnt repressor, H1= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI.

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Figure 5




Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor.

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Figure 6




Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).

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Figure 7




Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).

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Figure 8




Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI.

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Figure 9






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