Team:Lyon-INSA-ENS/Realisation/Protocols

From 2011.igem.org

(Difference between revisions)

Latest revision as of 16:08, 20 September 2011

General culture conditions

Antibiotics were used at the following concentrations :

Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :

LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water

Choose one of our protocols to read its description

All the protocols can be downloaded in pdf version by clicking on the corresponding logo :

. Biofilm quantification

. CaCl2 chemical transformation

. Fermentas digestion

. Ligation

. Macherey-Nagel miniprep kit

. Microscopy test

. Ozyme digestion

. Pouring of plates

. QIAGen midiprep kit

. QIAGen miniprep kit

. Storage of strains

. TSS chemical transformation

Before Starting First Week

@@ Line 2: / Line 2: @@
 {{Lyon-INSA-ENS/blocStyle}}
 {{INSA-Lyon/styletestaurelie}}
-{{Lyon-INSA-ENS/menuhorizontalRealisation}}
+{{Lyon-INSA-ENS/menuhorizontal}}
-{{Lyon-INSA-ENS/menuRealisationProtocoles}}
+<!--{{Lyon-INSA-ENS/menuNotebookProtocoles}}-->
+{{Lyon-INSA-ENS/menuNotebookVertical|Protocols = actif}}
 <html>
-<body>
+<div style="float : left; margin-top : -10px; margin-left : -200px">
+   <a href="https://2011.igem.org/Main_Page" >
+       <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
+   </a>
+</div>
-    <style type="text/css";>
+<body>
-.firstHeading{background-image: url(https://static.igem.org/mediawiki/2011/7/7f/Banniere_realisation.png);background-repeat: no repeat;}
-    </style>
 <div id="language";>
@@ Line 20: / Line 23: @@
      <br/> <br/>
-     <br/><br/><br/><br/><br/>
+    <br/> <br>
+        <p> <font color="green" size="5">
+              General culture conditions<br><HR>
+          </font></p>
+<br> <br><br> <br>
+<p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br><br>
+Ampicilin (Amp) : 100µg/mL <br>
+Chloramphenicol (Cm) : 20µg/mL <br>
+Spectinomycin (Spc) : 100µg/mL <br>
+Kanamycin (Kan) : 30µg/mL<br>
+The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br></p>
+<p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br>
+LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br>
+M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br>
+LB/2 : 50/50 (v/v) mix of LB and water<br>
+</p>
+    <br>
+    <br/> <br/>
+</div>
+<div class="contenugrand2";>
+     <br/> <br/>
+    <br/><br/><br/>
          <p> <font color="green" size="5">
@@ Line 28: / Line 59: @@
      <br> <br>
+    <br/> <br/>
+All the protocols can be downloaded in pdf version by clicking on the corresponding logo :
      <br/> <br/>
 <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a>
+<a href=" https://static.igem.org/mediawiki/2011/3/36/Biofilm_quantification.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
    </p>
-    <br/> <br/>
      <br/> <br/>
     <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a>
+<a href="https://static.igem.org/mediawiki/2011/f/fd/CaCl2_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
    </p>
-    <br/> <br/>
      <br/> <br/>
     <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a>
+<a href="https://static.igem.org/mediawiki/2011/6/65/Fermentas_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
    </p>
-    <br/> <br/>
      <br/> <br/>
   <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a>
+<a href="https://static.igem.org/mediawiki/2011/9/9d/Ligation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
-    <br/> <br/>
      <br/> <br/>
     <p> .
-<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli </a>
+<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a>
+<a href="https://static.igem.org/mediawiki/2011/4/4f/NucleoSpin_Plasmid_QuickPure.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
+   </p>
+    <br/> <br/><br/>
+<p>.
+<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy"> Microscopy test </a>
+<a href="https://static.igem.org/mediawiki/2011/d/d5/Microscopy_Test.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
+<br/>
     </p>
-    <br/> <br/>
      <br/> <br/>
 <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a>
+<a href="https://static.igem.org/mediawiki/2011/f/f8/Ozyme_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
      <br/> <br/>
-    <br/> <br/>
-   <p> .
-<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits </a>
+<p> .
+<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a>
+<a href="https://static.igem.org/mediawiki/2011/7/7f/Pouring_of_plates.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
      <br/> <br/>
+ <p> .
+<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a>
+<a href="https://static.igem.org/mediawiki/2011/5/53/QUIGen_midiprep_kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
+   </p>
      <br/> <br/>
     <p> .
-<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> Plasmid DNA purification using QIAprep® Spin Miniprep Kit</a>
+<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a>
+<a href="https://static.igem.org/mediawiki/2011/6/67/QUIGen_Miniprep_Kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
      <br/> <br/>
-    <br/> <br/>
 <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a>
+<a href="https://static.igem.org/mediawiki/2011/2/2a/Storage_of_strains.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
-    <br/> <br/>
      <br/> <br/>
      <p> .
 <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a>
+<a href="https://static.igem.org/mediawiki/2011/c/c7/TSS_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
      </p>
+<br><br><br><br><br><br><br>
+    <p>
+               <a  href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/BeforeStarting"/><font color="grey"><b>Before Starting</b></font></a>
+               <a  style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/Week1"/><font color="grey"><b>First Week</b></font></a>
+               <br/>
+    </p>
+<br><br><br>
 </div>
+</body>
 </html>
 {{Lyon-INSA-ENS/footer}}