Team:Virginia Tech

From 2011.igem.org

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[[Image:Virginia_Tech_logo.png|250px]]
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
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{| style=" background-color:#800000;" cellpadding="1" cellspacing="5" border="0" width="90%" align="center"
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!align="center"|[[Team:Virginia_Tech|<span style="color:orange;">Home</span>]]
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!align="center"|[[Team:Virginia_Tech/Team|<span style="color:orange;">Team</span>]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Virginia_Tech <span style="color:orange;">Official Team Profile</span>]
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!align="center"|[[Team:Virginia_Tech/Project|<span style="color:orange;">Project</span>]]
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!align="center"|[[Team:Virginia_Tech/Parts|<span style="color:orange;">Parts Submitted to the Registry</span>]]
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!align="center"|[[Team:Virginia_Tech/Notebook|<span style="color:orange;">Notebook</span>]]
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!align="center"|[[Team:Virginia_Tech/Safety|<span style="color:orange;">Safety</span>]]
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!align="center"|[[Team:Virginia_Tech/Attributions|<span style="color:orange;">Attributions</span>]]
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__NOTOC__
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|Team Members:
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<h3>Characterization of Fluorescent Reporters:</h3>
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Judith Eckman<br>
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[[File:kahn_fl_image.jpg|250 px|right]]
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Adam Grose<br>
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Fluorescent proteins have become ubiquitous tools for studying cellular processes, and are frequently used to visualize the dynamics of synthetic networks in cells. To be particularly effective for these applications, fluorescent proteins must feature fast maturation and degradation rates, and these rates must be well-characterized and documented. The 2011 VT iGEM team has worked to find and characterize fluorescent proteins and degradation tags that more quickly degrade them.  Here, we present chemical and mathematical models based on two parameters, maturation and degradation rates, which will hopefully contribute to more quantitative characterization and usage of fluorescent reporters in the future, and will inform future efforts in parts characterization. In conducting our experimentation, we tested different fluorescent proteins with degradation tags in <i>Escherichia coli </i> and <i>Saccharomyces cerevisiae</i> using automated fluorescent microscopy techniques, and then worked to determine a comprehensive, accurate mathematical basis for fluorescent protein characterization.
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Miranda Hagen<br>
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Brittany Harwell<br>
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Swetha Pasala<br>
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Mark Stewart<br>
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Meghan Savage<br>
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Hayley Schaefer<br>
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Loran Steinberger<br>
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Daniel Thorpe<br>
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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Our team consists of 10 undergraduates from Virginia Tech, UNC Chapel Hill, James Madison University, The University of Maryland, Bluefield State College, and The College of William & Mary.  We would like to thank Dr. Jean Peccoud and the Synthetic Biology Group at the Virginia Bioinformatics Institute for lab space, financial support, and guidance.
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!align="center"|[[Team:Virginia_Tech|Home]]
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!align="center"|[[Team:Virginia_Tech/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Virginia_Tech Official Team Profile]
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!align="center"|[[Team:Virginia_Tech/Project|Project]]
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!align="center"|[[Team:Virginia_Tech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Virginia_Tech/Modeling|Modeling]]
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!align="center"|[[Team:Virginia_Tech/Notebook|Notebook]]
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!align="center"|[[Team:Virginia_Tech/Safety|Safety]]
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!align="center"|[[Team:Virginia_Tech/Attributions|Attributions]]
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Latest revision as of 03:17, 29 September 2011


Division.png Virginia Tech logo.png Diatoms.png

Home Team Official Team Profile Project Parts Submitted to the Registry Notebook Safety Attributions


Characterization of Fluorescent Reporters:

Kahn fl image.jpg

Fluorescent proteins have become ubiquitous tools for studying cellular processes, and are frequently used to visualize the dynamics of synthetic networks in cells. To be particularly effective for these applications, fluorescent proteins must feature fast maturation and degradation rates, and these rates must be well-characterized and documented. The 2011 VT iGEM team has worked to find and characterize fluorescent proteins and degradation tags that more quickly degrade them. Here, we present chemical and mathematical models based on two parameters, maturation and degradation rates, which will hopefully contribute to more quantitative characterization and usage of fluorescent reporters in the future, and will inform future efforts in parts characterization. In conducting our experimentation, we tested different fluorescent proteins with degradation tags in Escherichia coli and Saccharomyces cerevisiae using automated fluorescent microscopy techniques, and then worked to determine a comprehensive, accurate mathematical basis for fluorescent protein characterization.


Our team consists of 10 undergraduates from Virginia Tech, UNC Chapel Hill, James Madison University, The University of Maryland, Bluefield State College, and The College of William & Mary. We would like to thank Dr. Jean Peccoud and the Synthetic Biology Group at the Virginia Bioinformatics Institute for lab space, financial support, and guidance.

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