From 2011.igem.org
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| - | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_HEAD}}
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| - | This is a placeholder. We should fill it in.
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| - | ==Construct Design==
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| - | ==Primer Design==
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| - | ==Assembly: first attempt==
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| - | ===PCR===
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| - | In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.
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| - | *We performed PCR using [[Team:Cambridge/Protocols/PCR#Reagents_used_in_PCR_reaction | Phusion Hot Start DNA Polymerase]] in 20 μl reaction volume.
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| - | ===Gibson Assembly===
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| - | ===Transformation===
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| - | ===Results===
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| - | ===Diagnostics===
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| - | ==Assembly: second attempt==
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| - | ===PCR===
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| - | ===Gibson Assembly===
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| - | ===Transformation===
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| - | ===Results===
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| - | ==What next?==
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| - | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}
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Latest revision as of 14:46, 15 September 2011
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