Team:Glasgow/sandbox

From 2011.igem.org

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<p><a href=https://2011.igem.org/Team:Glasgow/Results/PromoterLibrary>Results of Promoter Library + MCS</a>
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<p><a href="https://2011.igem.org/Team:Glasgow/Results:dispersal">Results for the Biofilm Dispersal</a>
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<p><a href="https://2011.igem.org/Team:Glasgow/Results:fixation">Results for the Biofilm Fixation - Encapsulation</a>
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<h1>Results</h1>
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Collated here are the results from numerous experiments that have been performed for the DISColi project. Detailed information about the biobricks we made is contained in links in the details of the experiment in which they are used.
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<h1>Meet the Team</h1>
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<p>We are a group of 6 second year and 3 third year Molecular and Cellular Biology and Genetics students lead by two PhD students and three Senior Lecturers from the University of Glasgow. This is the first time the University of Glasgow has entered a team to iGEM since 2007, when they won a gold medal and the environmental prize.
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<h4 class="tab_shift">Advisors</h4>
 
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Susan_Rosser"><img src="**image**" alt="Susan" width="160" height="120" /></a>
 
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Susan_Rosser">Susan Rosser</a></div></td>
 
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Rainer_Breitling"><img src="https://static.igem.org/mediawiki/2011/0/07/Rainer.jpg" alt="Rainer" width="160" height="120" /></a>
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<p>Some of the application ideas we had for our project included:</p>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Rainer_Breitling">Rainer Breitling</a></div></td>
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<ul><p>Creation of medicinal products in extreme environments:</p>
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<ul><p>As light is an ubiquitous resource, it makes sense to have light-controlled machinery inside bacteria. This would mean that creation of desired products - such as medicines or enzymes - wouldn't require the expensive transport of chemicals to trigger these responses. This is particularly relevant in places such as space, where transport of any material is extremely expensive. Imagine bringing a small vial of bacteria up in a rocket and having it self-assemble into a complex piece of machinery by simply adding re-cycled growth media and light!</p></ul>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Joe_Gray"><img src="**image**" alt="Joe" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Joe_Gray">Joe Gray</a></div></td>
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<ul><p>Micro-engineering:</p>
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<ul><p>Through target-specific encapsulation and dispersal machinery, partially permeable biofilm could be formed into areas which allow either a rapid or slow movement of fluids through them at specific points. On top of this, any individual bacteria in the formed matrix could be triggered to produce a specific chemical or enzyme at any time allowing formation of a microscopic chemical engineering production line. We have performed modelling on the diffusion gradients of all our biobricks to generate approximate applicable resolutions.</p></ul>
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</ul>
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<ul><p>Industrial Cleaning of Bio-reactors:</p>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Christine_Merrick"><img src="https://static.igem.org/mediawiki/2011/9/9c/Christine.jpg" alt="Christine" width="160" height="120" /></a>
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<ul><p>Biofilm formation is a constant menace in large-scale biotechnology, where bacteria left resting in a reactor form a biofilm which resists conventional cleaning methods. They also reduce reactor lifespan through corrosion and reduces reaction efficiency by insulating heat or absorbing nutrients. We have created several biobricks which trigger biofilm dispersal. By joining these to a specifically active promoter - either a light, chemically, or temperature activated promoter - and inserting them into the bacterial machinery. Once the bio-reaction is completed, the tank can be treated in such a manner that the biofilm dispersal mechanisms become active - thus eliminating the biofilm and increasing the profitability of the Biotechnology industry.</p></ul>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Christine_Merrick">Christine Merrick</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Stephanie_Evans"><img src="https://static.igem.org/mediawiki/2011/5/59/Stephanie.jpg" alt="Stephanie" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Stephanie_Evans">Stephanie Evans</a></div></td>
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<h4 class="tab_shift">Undergraduates</h4>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels"><img src="https://static.igem.org/mediawiki/2011/4/44/Andrew.jpg" alt="Andrew" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels">Andrew Landels</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Chris_Wood"><img src="https://static.igem.org/mediawiki/2011/d/dd/Chris.jpg" alt="Chris" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Chris_Wood">Chris Wood</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert"><img src="https://static.igem.org/mediawiki/2011/2/21/Ellin.png" alt="Ellin" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert">Ellin Hillert</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell"><img src="https://static.igem.org/mediawiki/2011/4/46/Emma.png" alt="Emma" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell">Emma Campbell</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Hannah_Ralph"><img src="https://static.igem.org/mediawiki/2011/0/0d/Hannah.png" alt="Hannah" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Hannah_Ralph">Hannah Ralph</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz"><img src="https://static.igem.org/mediawiki/2011/d/d5/Michal.png" alt="Michal" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz">Michal Przydacz</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone"><img src="https://static.igem.org/mediawiki/2011/1/12/Pietro.jpg" alt="Pietro" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone">Pietro Ridone</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Scott_Wood"><img src="https://static.igem.org/mediawiki/2011/b/be/Scott.jpg" alt="Scott" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Scott_Wood">Scott Wood</a></div></td>
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  <td><div class="image"><a href="https://2011.igem.org/Team:Glasgow/Team/Stewart_O'Neill"><img src="https://static.igem.org/mediawiki/2011/0/05/Stewart.jpg" alt="Stewart" width="160" height="120" /></a>
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<div class="name"><a href="https://2011.igem.org/Team:Glasgow/Team/Stewart_O'Neill">Stewart O'Neill</a></div></td>
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<h2>What we did</h2>
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<p>(Provide proper attribution for all work)</p>
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<h2>Where we're from</h2>
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We all study at the <a href="http://www.gla.ac.uk">University of Glasgow</a> in Scotland's largest city.<br />
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The University was founded in 1451, and apart from being generally amazing it is most notable for its gorgeous neo-gothic main building designed by Sir George Gilbert Scott. With its spire and quadrangles it is enough to make anyone feel like a student of Witchcraft and Wizardry.<br />
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The University was based in <a href="http://www.glasgowcathedral.org.uk/">Glasgow Cathedral</a> until 1460. It then moved to Glasgow High Street, where it operated for the next 400 years. When the University outgrew its facilities there it relocated to Gillmorehill, to its new, specially built campus. The campus, where over 23,000 students attend, currently comprises of 104 buildings.<br />
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The University library holds over 2.5 million books on 12 floors, making it one of the largest libraries in Europe.<br />
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[[File:University.jpg|200px|thumb|left|The Main Building at Gillmorehill]]
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[[File:University2.jpg|200px|thumb|left]]
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[[File:Cloisters.jpg|200px|thumb|left|Cloisters in the Main Building]]
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Glasgow itself is a great city with a vibrant nightlife and a broad range of more cultural activities to choose from. Many museums are free for anyone and always worth a look. For Example, the <a href="http://en.wikipedia.org/wiki/Kelvingrove_Art_Gallery_and_Museum">Kelvingrove Museum</a> offers a slightly random collection of natural history and art, and is also architecturally very interesting. Additionally it is a great place for anybody who would like to learn more about the history of Glasgow.<br />
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[[File:Kelvincone.jpg|200px|thumb|left|Kelvin and his Glasgow Hat]]
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[[File:Kelvingrove.jpg|200px|thumb|left|The Kelvingrove]]
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[[File:Auchentoshan.jpg|200px|thumb|left|Auchentoshan]]
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<img src="https://static.igem.org/mediawiki/2011/7/7c/Patternformation.png" width="800" height="725" alt="Pattern Formation" usemap="#patternformation" />
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  <area shape="rect" coords="696,443,743,715" alt="Product Selection" href="productselection.htm" />
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  <area shape="rect" coords="48,99,219,205" alt="Pattern Forming Device" href="https://2011.igem.org/Team:Glasgow/Pattern_Forming_Device" />
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  <area shape="rect" coords="48,244,220,349" alt="Structure Fixing Device" href="https://2011.igem.org/Team:Glasgow/Structure_Fixing_Device" />
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  <area shape="rect" coords="591,55,763,161" alt="Product 1 Generating Device" href="https://2011.igem.org/Team:Glasgow/Product_1_Generating_Device" />
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  <area shape="rect" coords="591,173,763,280" alt="Product 2 Generating Device" href="https://2011.igem.org/Team:Glasgow/Product_2_Generating_Device" />
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Latest revision as of 17:37, 21 September 2011

Results of Promoter Library + MCS

Results for the Biofilm Dispersal

Results for the Biofilm Fixation - Encapsulation

Results

Collated here are the results from numerous experiments that have been performed for the DISColi project. Detailed information about the biobricks we made is contained in links in the details of the experiment in which they are used.

Some of the application ideas we had for our project included:

    Creation of medicinal products in extreme environments:

      As light is an ubiquitous resource, it makes sense to have light-controlled machinery inside bacteria. This would mean that creation of desired products - such as medicines or enzymes - wouldn't require the expensive transport of chemicals to trigger these responses. This is particularly relevant in places such as space, where transport of any material is extremely expensive. Imagine bringing a small vial of bacteria up in a rocket and having it self-assemble into a complex piece of machinery by simply adding re-cycled growth media and light!

    Micro-engineering:

      Through target-specific encapsulation and dispersal machinery, partially permeable biofilm could be formed into areas which allow either a rapid or slow movement of fluids through them at specific points. On top of this, any individual bacteria in the formed matrix could be triggered to produce a specific chemical or enzyme at any time allowing formation of a microscopic chemical engineering production line. We have performed modelling on the diffusion gradients of all our biobricks to generate approximate applicable resolutions.

    Industrial Cleaning of Bio-reactors:

      Biofilm formation is a constant menace in large-scale biotechnology, where bacteria left resting in a reactor form a biofilm which resists conventional cleaning methods. They also reduce reactor lifespan through corrosion and reduces reaction efficiency by insulating heat or absorbing nutrients. We have created several biobricks which trigger biofilm dispersal. By joining these to a specifically active promoter - either a light, chemically, or temperature activated promoter - and inserting them into the bacterial machinery. Once the bio-reaction is completed, the tank can be treated in such a manner that the biofilm dispersal mechanisms become active - thus eliminating the biofilm and increasing the profitability of the Biotechnology industry.

Retrieved from "http://2011.igem.org/Team:Glasgow/sandbox"