Copenhagen/16 August 2011

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'''Labwork'''
'''Labwork'''
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* Checked the TLC from yesterday, the result was not good. There was no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
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* Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
-
* Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See the protocole [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Expression & Purification in BL21]]
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* Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Expression & Purification in BL21]]

Latest revision as of 07:54, 17 August 2011

Tuesday

Labwork

  • Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
  • User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
  • Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol Expression & Purification in BL21


Other work

  • Worked on the poster
  • Worked on the illustrations
  • Searched for hotel in Amsterdam


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