Copenhagen/16 August 2011
From 2011.igem.org
(Difference between revisions)
(→Tuesday) |
|||
(One intermediate revision not shown) | |||
Line 3: | Line 3: | ||
'''Labwork''' | '''Labwork''' | ||
- | * Checked the TLC from yesterday, the result | + | * Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2. |
* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again. | * User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again. | ||
- | * Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See | + | * Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Expression & Purification in BL21]] |
Latest revision as of 07:54, 17 August 2011
Tuesday
Labwork
- Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
- User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
- Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol Expression & Purification in BL21
Other work
- Worked on the poster
- Worked on the illustrations
- Searched for hotel in Amsterdam
Back to Notebook