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Copenhagen/16 August 2011
From 2011.igem.org
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'''Labwork''' | '''Labwork''' | ||
| - | * Checked the TLC from yesterday, the result | + | * Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2. |
* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again. | * User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again. | ||
| - | * Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1,2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. | + | * Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Expression & Purification in BL21]] |
| - | |Expression & Purification in BL21]] | + | |
| - | + | ||
| - | + | ||
Latest revision as of 07:54, 17 August 2011
Tuesday
Labwork
- Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
- User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
- Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol Expression & Purification in BL21
Other work
- Worked on the poster
- Worked on the illustrations
- Searched for hotel in Amsterdam
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