Team:UT Dallas/NotebookWeek2

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           <h2><span> Week 2</span></h2>
           <h2><span> Week 2</span></h2>
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           <p><font size = "5" face= "arial" color = "#184D79"><b>July 18-</b></font>
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              <blockquote><li type = "circle">Colony grew, negative control didn’t<li type = "circle">Transfomation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all carb)<li type = "circle">Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol<li type = "circle">Miniprep<li type = "circle">Dissolving and diluting primers (10 pmol/µL)<li type = "circle">PCR and gel electrophoresis and purification<li type = "circle">Digestion for 1 hour and 30 minutes at 37 degrees Celsius<li type = "circle">Gel Electrophoresis and Purification <li type = "circle">Digestion and ligation</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 19-</b></font>
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              <blockquote><li type = "circle">Transformation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 20-</b></font>
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              <blockquote><li type = "circle">Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature<li type = "circle">Transformation of Photo and YFP 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all chlora)<li type = "circle">Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 21-</b></font>
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              <blockquote><li type = "circle">Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells<li type = "circle">Miniprep<li type = "circle">Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Test digestion for 1 hour and 20 minutes a 37 degrees Celsius<li type = "circle">- Test digestion for 1 hour and 20 minutes a 37 degrees Celsius</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 22-</b></font>
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              <blockquote><li type = "circle">Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells<li type = "circle">Miniprep<li type = "circle">Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol<li type = "circle">Dissolving and Diluting primers<li type = "circle">Test digestion and gel electrophoresis </blockquote>
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       <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
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       <h2><span><font size="5" face="verdana">Notebook</span></font></h2>
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       <h2><span><font size="5" face="arial">Notebook</span></font></h2>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>

Latest revision as of 02:12, 3 September 2011

biz solution

Week 2

July 18-

  • Colony grew, negative control didn’t
  • Transfomation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all carb)
  • Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol
  • Miniprep
  • Dissolving and diluting primers (10 pmol/µL)
  • PCR and gel electrophoresis and purification
  • Digestion for 1 hour and 30 minutes at 37 degrees Celsius
  • Gel Electrophoresis and Purification
  • Digestion and ligation

    • July 19-

  • Transformation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight

    • July 20-

  • Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature
  • Transformation of Photo and YFP 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all chlora)
  • Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513

    • July 21-

  • Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Test digestion for 1 hour and 20 minutes a 37 degrees Celsius
  • - Test digestion for 1 hour and 20 minutes a 37 degrees Celsius

    • July 22-

  • Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol
  • Dissolving and Diluting primers
  • Test digestion and gel electrophoresis

    • Weeks 1-12

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    Notebook

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