Team:MIT

From 2011.igem.org

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        <h2>Introduction</h2>
 
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        <p>Our team is conveniently located alongside the lab of Tom Knight, the father of
 
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synthetic biology, and iGEM headquarters, putting us in a unique position to be
 
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able to interact on a daily basis with the talented staff running iGEM.
 
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<h3>Tissues by Design</h3>
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<p>Our project focuses on tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators) with genetic circuits. To accomplish this, we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.</p>
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<p><img src="https://static.igem.org/mediawiki/2011/5/51/Simulation.jpg" style="max-width:800px; margin-right:10px;"/></p></br>
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<p>Above is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.</p>
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<p>Specifically, for cell-cell signaling, we developed a modular juxtacrine platform, using Notch and Delta proteins. For intracellular information processing circuits, as a proof of concept, we build a 2-input AND gate. For cell-cell adhesion, the final output of our system, we used cadherin.
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Below is an animation depicting our project components. The cell-cell signaling of Notch-Delta interaction leads to the cleavage of the Notch intracellular domain, which enters the nucleus and after logic processing, expresses cadherins, which cause cells to adhere to similarly expressing cells.
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<div align="center"><iframe width="400" height="300" src="http://www.youtube.com/embed/rGOB0gMxf_8" frameborder="0" allowfullscreen></iframe></div>
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We developed software tools to model the behavior of our system. Below is a sample of a simulation of cells with genetic circuits and how they differentiate.
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<div align="center"><iframe width="400" height="300" src="http://www.youtube.com/embed/dbz4VegsJOw?rel=0&amp;hd=1" frameborder="0" allowfullscreen></iframe>
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             <h3>Partners</h3>
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             <h3>Sponsors</h3>
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        <li><a href="http://www.bbn.com/"><img src='https://static.igem.org/mediawiki/2011/0/03/Mit-bbn.jpg' /></a></li>
        <li><a href="http://www.bbn.com/"><img src='https://static.igem.org/mediawiki/2011/0/03/Mit-bbn.jpg' /></a></li>
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        <li><a href="http://www.genewiz.com"><img src='https://static.igem.org/mediawiki/2011/3/33/Mit-genewiz.jpg' /></a></li>
        <li><a href="http://www.genewiz.com"><img src='https://static.igem.org/mediawiki/2011/3/33/Mit-genewiz.jpg' /></a></li>
        <li><a href="http://www.neb.com"><img src='https://static.igem.org/mediawiki/2011/4/4d/Mit-neb.jpg' /></a></li>
        <li><a href="http://www.neb.com"><img src='https://static.igem.org/mediawiki/2011/4/4d/Mit-neb.jpg' /></a></li>
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        <li><a href="http://www.embitec.com"><img src='https://static.igem.org/mediawiki/2011/7/75/Mit-embitec.jpg' /></a></li>
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        <li><a href="http://www.embitec.com"><img src='https://static.igem.org/mediawiki/2011/7/75/Mit-embitec.jpg' style="width:175px" /></a></li>
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                <li><a href="http://ebics.net"><img src='https://static.igem.org/mediawiki/igem.org/0/0d/EBICS_logo.JPG' style="width:175px"></a></li>
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                <li><a href="http://ginkgobioworks.com/"><img src='http://ginkgobioworks.com/images/ginkgobioworks_logo.png' style="width:175px; "></a></li>
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                <li><a href="https://2011.igem.org/Main_Page"><img src='https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png' style="width:175px;"></li>
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             <h3>Gold Sponsors</h3>
             <h3>Gold Sponsors</h3>
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    <li><a href="http://www.emdchemicals.com"><img src='https://static.igem.org/mediawiki/2011/2/2f/Mit-gold-emd.jpg' /></a></li>
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    <li><a href="http://www.emdchemicals.com"><img src='https://static.igem.org/mediawiki/2011/e/ee/Gold_emdmillipore.jpg' /></a></li>
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<li><a href="http://www.millipore.com"><img src='https://static.igem.org/mediawiki/2011/9/9f/Mit-gold-millipore.jpg' /></a></li>
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<li><a href="http://www.eecs.mit.edu/"><img src='https://static.igem.org/mediawiki/2011/2/22/Mit-eecs.jpg' /></a></li>
<li><a href="http://www.eecs.mit.edu/"><img src='https://static.igem.org/mediawiki/2011/2/22/Mit-eecs.jpg' /></a></li>
<li><a href="http://web.mit.edu/be/"><img src='https://static.igem.org/mediawiki/2011/a/a7/Mit-be.jpg' /></a></li>
<li><a href="http://web.mit.edu/be/"><img src='https://static.igem.org/mediawiki/2011/a/a7/Mit-be.jpg' /></a></li>
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                <li><a href="http://web.mit.edu/biology/www/"><img src='https://static.igem.org/mediawiki/2011/b/bf/Mitbiologo.jpg' /></a></li>
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<h2>History</h2>
 
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<p>The MIT iGEM team is coming off its most successful year in program
 
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history where, in addition to fulfilling the requirements to receive a gold medal,
 
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the team earned the distinction of Best Manufacturing Project and also was
 
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awarded the iGEMers Prize (voted best project by other iGEM teams). Both the
 
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scope of possible projects and resources available to the MIT team was greatly
 
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expanded with the arrival of Professor Ron Weiss, who coached award-winning
 
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iGEM teams at Princeton since the beginning of the competition. </p><br />
 
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<p>Along with
 
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Prof. Weiss, the 2010 team was under the leadership and aided by numerous
 
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professors and graduate instructors who will be returning this year to continue
 
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leading the program to success. As a team, we are proud of our continued
 
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success, our rich iGEM history, and the strong relationships we are able to
 
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build and maintain with many of the leading researchers in the field of synthetic
 
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biology.</p>
 
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<a href="http://twitter.com/share" class="twitter-share-button" data-count="none" >Tweet</a><script type="text/javascript" src="http://platform.twitter.com/widgets.js"></script> <br />
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<a href="http://twitter.com/MIT_iGEM" class="twitter-follow-button">Follow @MIT_iGEM</a>
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        <h2>Facebook</h2>
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<h2>Contact Us</h2>
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<p><span class="green">Email:</span><a href="index.html"> igem2011@mit.edu</a></p>
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<script type="text/javascript" src="http://twitter.com/javascripts/blogger.js"></script>
 
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<script type="text/javascript" src="http://twitter.com/statuses/user_timeline/MIT_iGEM.json?callback=twitterCallback2&count=4"></script>
 
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Latest revision as of 04:03, 29 October 2011

Tissues by Design

Our project focuses on tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators) with genetic circuits. To accomplish this, we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.



Above is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.

Specifically, for cell-cell signaling, we developed a modular juxtacrine platform, using Notch and Delta proteins. For intracellular information processing circuits, as a proof of concept, we build a 2-input AND gate. For cell-cell adhesion, the final output of our system, we used cadherin. Below is an animation depicting our project components. The cell-cell signaling of Notch-Delta interaction leads to the cleavage of the Notch intracellular domain, which enters the nucleus and after logic processing, expresses cadherins, which cause cells to adhere to similarly expressing cells.



We developed software tools to model the behavior of our system. Below is a sample of a simulation of cells with genetic circuits and how they differentiate.

Sponsors

MIT Departments

Retrieved from "http://2011.igem.org/Team:MIT"