Team:Fatih Turkey/Project

From 2011.igem.org

(Difference between revisions)
 
(54 intermediate revisions not shown)
Line 1: Line 1:
<html xmlns="http://www.w3.org/1999/xhtml">
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
-
<title>Fatih_Turkey</title>
+
<title>deneme baslik</title>
-
<style type="text/css">
+
<style>
-
<!--
+
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
 +
  display:none;
 +
}
 +
#menubar{
 +
background-color: transparent;
 +
font-size: 85%;
 +
top: 10px;
 +
position: absolute;
 +
margin-top: -12px;
 +
}
 +
 
 +
div#content{
 +
padding: 0 !important;
 +
background: transparent;
 +
border: none !important;
 +
width: auto;
 +
}
 +
 
 +
#menubar li a {
 +
color: #FFFFFF;
 +
background-color: transparent;
 +
}
 +
#globalWrapper{
 +
margin-top:5px;
 +
}
 +
#top-section{
 +
height: 20px !important;
 +
border: none !important;
 +
}
body {
body {
-
background-color: #FFFFFF;
+
font-size: 12px;
 +
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;
 +
color: #444;
 +
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');
}
}
-
.style3 {color: #FFFFFF}
+
body,p,pre,div,span{
-
body,td,th {
+
line-height: 160%;
-
font-family: Verdana, Arial, Helvetica, sans-serif;
+
color: #333;
-
color: #FFFFFF;
+
}
}
-
a:link {
+
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,
 +
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td
 +
{
 +
margin:0;
 +
padding:0;
 +
border:0;
 +
font-weight:inherit;
 +
font-style:inherit;
 +
font-family:inherit;
 +
vertical-align:baseline;
 +
}
 +
 
 +
em{
 +
font-style:italic;
 +
}
 +
table{
 +
margin-top: 15px;
 +
margin-bottom: 15px;
 +
}
 +
hr{
 +
border: 0;
 +
color: #eee;
 +
background-color: #eee;
 +
height: 1px;
 +
margin-bottom: 20px;
 +
}
 +
img.centered {
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
img.alignnone {
 +
margin: 0px 0px 5px 5px;
 +
display: inline;
 +
}
 +
img.alignright {
 +
margin: 0px 0px 5px 10px;
 +
display: inline;
 +
}
 +
 
 +
img.alignleft {
 +
margin: 0px 10px 5px 0px;
 +
display: inline;
 +
}
 +
.alignright {
 +
float: right;
 +
}
 +
 
 +
.alignleft {
 +
float: left;
 +
}
 +
.aligncenter{
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
h1,h2,h3,h4,h5,h6{
 +
  margin-top: 15px;
 +
  margin-bottom: 15px ;
 +
  color: #333;
 +
  line-height: 1.5em;
 +
}
 +
h1 {
 +
font-size: 30px;
 +
}
 +
h2 {
 +
font-size: 25px;
 +
}
 +
h3 {
 +
font-size: 20px;
 +
}
 +
h4 {
 +
font-size: 18px;
 +
}
 +
h5 {
 +
font-size: 16px;
 +
}
 +
 
 +
h6 {
 +
font-size: 15px;
 +
}
 +
a{
text-decoration: none;
text-decoration: none;
}
}
-
a:visited {
+
a{
 +
color: #2f86c4;
 +
}
 +
a:hover{
 +
color: #4172ab;
 +
}
 +
#header .logo{
 +
margin-top: 41px;
 +
margin-left: 40px;
 +
}
 +
#footer{
 +
background: #707070;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
width: 988px;
 +
margin: 0px auto 0px;
 +
overflow: hidden;
 +
}
 +
#footer div{
 +
color: #bbb;
 +
}
 +
#footer a{
 +
color: #c4c5cc;
text-decoration: none;
text-decoration: none;
}
}
-
a:hover {
+
#footer a:hover{
 +
color: #666;
 +
}
 +
 
 +
.footer-sh-wrapper {
 +
    height: 22px;
 +
    margin: auto;
 +
    position: relative;
 +
    width: 990px;
 +
}
 +
.footer-sh-left {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    left: 0;
 +
    position: absolute;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
.footer-sh {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;
 +
    bottom: 0;
 +
    height: 22px;
 +
    left: 10px;
 +
    position: absolute;
 +
    width: 970px;
 +
}
 +
.footer-sh-right {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    position: absolute;
 +
    right: 0;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
 
 +
.copyright-open{
 +
width: 988px;
 +
height: 0px;
 +
margin:auto;
 +
display: block-inline;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
color: #333333;
 +
}
 +
#header-whole{
 +
 
 +
position: relative;
 +
z-index: 200;
 +
width: 980px;
 +
margin: 0 auto;
 +
}
 +
#header{
 +
height: 100px;
 +
clear: both;
 +
background: #f9f9f9;
 +
margin: 0 auto;
 +
    width: 970px;
 +
}
 +
#header .logo{
 +
float: left;
 +
margin-top: 15px;
 +
margin-left: 20px;
 +
}
 +
 
 +
#container{
 +
width: 970px;
 +
margin: 0px auto 0px;
 +
background: none repeat scroll 0 0 #F9F9F9;
 +
}
 +
.copyright-open{
 +
border-top: 1px #a8a8a8 solid;
 +
border-bottom: 1px #888 solid;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
}
 +
.nav-wrapper{
 +
margin: 0px 90px 0px 0px;
 +
float:right;
 +
}
 +
.ddsmoothmenu ul{
 +
padding: 0px;
 +
list-style-type: none;
 +
background: #666666;
 +
}
 +
.ddsmoothmenu ul li{
 +
position: relative;
 +
display: block;
 +
float: left;
 +
}
 +
.ddsmoothmenu ul li a{
 +
line-height: 140%;
 +
overflow: hidden;
 +
text-align: left;
 +
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;
 +
word-spacing: 1px;
 +
font-size: 14px;
 +
font-weight: normal;
 +
display: block; /*background of menu items (default state)*/
 +
padding: 34px 20px 10px 17px;
text-decoration: none;
text-decoration: none;
}
}
-
a:active {
+
.ddsmoothmenu ul li a span {
 +
font-weight: normal;
 +
color: #888;
 +
font-size: 10px;
 +
clear: both;
 +
display:block;
 +
width: 60px;
 +
}
 +
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/
 +
display: inline-block;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{
 +
color: #333;
 +
}
 +
 
 +
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #888;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:hover{
 +
color: #888;
 +
}
 +
 +
/*1st sub level menu*/
 +
.ddsmoothmenu ul li ul{
 +
position: absolute;
 +
left: 0;
 +
visibility: hidden;
 +
display: none; /*collapse all sub menus to begin with*/
 +
border: 1px #222 solid;
 +
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
}
 +
 
 +
/*Sub level menu list items (undo style from Top level List Items)*/
 +
.ddsmoothmenu ul li ul li{
 +
text-align:left;
 +
float: none;
 +
background: url('img/bg-menu2.png');
 +
}
 +
 
 +
.ddsmoothmenu ul li ul li a{
 +
color: #fff;
 +
font-size: 12px;
 +
}
 +
.ddsmoothmenu ul li ul li  a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:hover{
 +
color: #ffc;
 +
}
 +
.ddsmoothmenu ul li ul li ul{
 +
top: 0;
 +
}
 +
 
 +
/* Sub level menu links style */
 +
.ddsmoothmenu ul li ul li a{
 +
width: 160px; /*width of sub menus*/
 +
padding: 0px 0px 0px 10px;
 +
height: 38px;
 +
line-height: 320%;
 +
border-left: 1px #555 solid;
 +
overflow: hidden;
 +
}
 +
 
 +
/* Holly Hack for IE \*/
 +
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/
 +
 
 +
.copyright{
 +
width: 930px;
 +
color: #959595;
 +
background: #eee;
 +
clear: both;
 +
line-height: 190%;
 +
text-align: right;
 +
padding: 10px 20px 10px;
 +
margin: 0px auto 0px;
 +
}
 +
.copyright a{
 +
color: #bbb;
text-decoration: none;
text-decoration: none;
}
}
-
.style4 {font-size: 12px}
+
.copyright a:hover{
-
.style5 {font-size: 24px}
+
color: #ccc;
-
-->
+
}
-
</style></head>
+
.bar-title-whole{
 +
overflow: visible;
 +
position: relative;
 +
width: 990px;
 +
margin: auto;
 +
z-index: 10;
 +
}
 +
.bar-title{
 +
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;
 +
border-left: 1px #8a8a8a solid;
 +
border-right: 1px #8a8a8a solid;
 +
width: 918px;
 +
height: 122px;
 +
margin: auto;
 +
overflow: hidden;
 +
padding-left: 35px;
 +
padding-right: 35px;
 +
color: #ddd;
 +
line-height: 150%;
 +
font-size: 12px;
 +
 +
position: relative;
 +
z-index: 10;
 +
}
 +
 +
.bar-title h2{
 +
color: #fff;
 +
margin-top: 15px;
 +
margin-bottom: 30px !important;
 +
}
 +
.bar-title h5{
 +
color: #eee;
 +
}
 +
.bar-title-sh-left{
 +
width: 10px;
 +
height: 112px;
 +
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 0px;
 +
}
 +
.bar-title-sh{
 +
width: 970px;
 +
height: 8px;
 +
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 10px;
 +
}
 +
.bar-title-sh-right{
 +
width: 10px;
 +
height: 112px;
 +
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
right: 0px;
 +
}
 +
#page{
 +
    width: 970px;
 +
    margin: 0px auto 0px;
 +
    overflow: hidden;
 +
    background: #f9f9f9;
 +
   
 +
}
 +
 +
#page .page-wrapper{
 +
margin: 15px 30px 0px 30px;
 +
width: 910px;
 +
overflow: hidden;
 +
float: left;
 +
}
 +
#page .page-wrapper-right{
 +
margin: 30px 40px 0px 25px;
 +
width: 935px;
 +
overflow: hidden;
 +
float: right;
 +
}
 +
#page .title-wrapper h1{
 +
margin-top: 0.5em;
 +
margin-bottom: 0.3em;
 +
}
 +
#page .meta-left-full{
 +
margin: 15px 0px 5px 0px;
 +
overflow: hidden;
 +
}
 +
#page .meta-left-button{
 +
margin: 5px 0px 60px 0px;
 +
overflow: hidden;
 +
}
 +
#page .w600{
 +
width: 600px;
 +
}
 +
 +
</style>
 +
 +
 +
</head>
<body>
<body>
-
<table width="975" border="0" align="center">
+
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script>
-
  <tr>
+
 
-
    <td width="969" height="236" align="center" valign="middle" background="https://static.igem.org/mediawiki/2011/0/02/Renkli.jpg">&nbsp;</td>
+
<script>
-
  </tr>
+
$(document).ready(
-
  <tr>
+
ddsmoothmenu.init({
-
    <td height="53" bgcolor="#FFFFFF"><table width="969" border="0" cellspacing="10" bgcolor="#FFFFFF">
+
mainmenuid: "topmenu",
-
        <tr>
+
orientation: 'h',
-
          <td width="223" align="center" valign="middle" bgcolor="#990000"><a href="https://2011.igem.org/Team:Fatih_Turkey"><span class="style3">HOME</span></a></td>
+
classname: 'ddsmoothmenu',
-
          <td width="225" align="center" valign="middle" bgcolor="#695E0E"><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_town"><span class="style3">CANVAS&nbsp; TOWN</span></a></td>
+
contentsource: "markup"
-
          <td width="281" align="center" valign="middle" bgcolor="#1D3551"><a href="therainbowgraveyard.html"><span class="style3">THE RAINBOW GRAVEYARD</span></a></td>
+
}));
-
          <td width="182" align="center" valign="middle" bgcolor="#960C47"><a href="notebook.html"><span class="style3">&nbsp;NOTEBOOK</span></a></td>
+
 
-
        </tr>
+
jQuery(window).load(function() {
-
        <tr>
+
setTimeout(function(){
-
          <td height="20" colspan="4" align="center" valign="middle" bgcolor="#1D3551"><a href="project.html"><span class="style3">PROJECT</span></a></td>
+
jQuery('#slider').nivoSlider({
-
        </tr>
+
pauseTime:4000,
-
    </table></td>
+
animSpeed:600,
-
  </tr>
+
captionOpacity: 0.8,
-
  <tr>
+
pauseOnHover:true,
-
    <td height="778" align="center" valign="top" bgcolor="#FFFFFF"><table width="614" height="75" border="0" cellpadding="20" cellspacing="10">
+
directionNav:true,
-
      <tr>
+
directionNavHide:true,
-
        <td width="231"><a href="anti-lps.html"><img src="https://static.igem.org/mediawiki/2011/1/16/Anti_lps_project.jpg" width="200" height="250" /></a></td>
+
controlNavThumbs:true,
-
        <td width="219"><a href="reflectin.html"><img src="https://static.igem.org/mediawiki/2011/3/33/Reflectin_project.jpg" width="200" height="250" /></a></td>
+
controlNavThumbsFromRel:true,
-
        <td width="200"><a href="biofilm.html"><img src="https://static.igem.org/mediawiki/2011/1/1c/Biofilm_project.jpg" width="200" height="250" /></a></td>
+
});
-
      </tr>
+
}, 1000),
-
    </table>
+
setTimeout(function(){
-
      <p>&nbsp;</p>
+
/* For slider shortcode */
-
      <p><a href="conclusio.html"><img src="https://static.igem.org/mediawiki/2011/0/04/Conc-proj.jpg" width="150" height="188" /></a>&nbsp;&nbsp; &nbsp;&nbsp;&nbsp; <a href="description.html"><img src="https://static.igem.org/mediawiki/2011/5/55/Descrip-proj.jpg" width="150" height="188" /></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <a href="discuccion.html"><img src="https://static.igem.org/mediawiki/2011/8/8f/Dis-proj.jpg" width="150" height="188" /></a></p>
+
jQuery('#slider2').nivoSlider({
-
      <p>&nbsp;</p>
+
pauseTime:4000,
-
       <p><a href="futureplan.html"><img src="https://static.igem.org/mediawiki/2011/c/ce/Future-proj.jpg" width="150" height="188" /></a>&nbsp;&nbsp;&nbsp; &nbsp;&nbsp; <a href="planning.html"><img src="
+
animSpeed:600,
-
https://static.igem.org/mediawiki/2011/a/a3/Plann-proj.jpg" width="150" height="188" /></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <a href="references.html"><img src="https://static.igem.org/mediawiki/2011/a/a8/Ref-proj.jpg" width="150" height="188" /></a></p>
+
captionOpacity: 0.8,
-
      <p>&nbsp;</p>
+
pauseOnHover:true,
-
      <p>&nbsp;</p>   </td>
+
directionNavHide:true });
-
  </tr>
+
}, 1000);
-
</table>
+
 +
});
 +
</script>
 +
 
 +
<div class="body-2">
 +
<!-- Header -->
 +
<div id="header-whole">
 +
<div id="header">
 +
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a>
 +
<!-- Logo -->
 +
<div>
 +
<div class="logo">
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey">
 +
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png">
 +
</a>
 +
</div>
 +
</div>
 +
<!-- Menu -->
 +
<div class="nav-wrapper">
 +
<div class="nav-main">
 +
<div id="topmenu" class="ddsmoothmenu">
 +
<ul id="menu-main" class="menu">
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a>
 +
</li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a>
 +
<ul>
 +
                                                                                <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li>
 +
                                                                                  <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li>
 +
                                                                                  <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
 +
                                                                                <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li>
 +
</ul>
 +
</li>
 +
                                                            </ul>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- End Header -->
 +
<div class="bar-title-whole">
 +
  <div class="bar-title">
 +
      <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2>
 +
      <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Overall Project</h5>
 +
    </div>
 +
  <div class="bar-title-sh-left"></div>
 +
  <div class="bar-title-sh"></div>
 +
  <div class="bar-title-sh-right"></div>
 +
        </div>
 +
</div>
 +
<div id="container">
 +
<div id="page">
 +
<div class="page-wrapper">
 +
<!-- post container -->
 +
<div>
 +
<div class="meta-left-full">
 +
<h1>The Rainbow Graveyard</h1>
 +
<center><img src="https://static.igem.org/mediawiki/2011/5/51/Growth_inh..png" width="400" /></center>
 +
<br>
 +
 
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">E. Coli is one of the gram-negative bacteria and most of their types are harmless. However some of E.Coli types such as enterohaemorrhagic E.Coli (EHEC) can cause serious diseases.</p>
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">In our project we designed an innovative model to prevent gram-negative growth and infection. In our model, to prevent E.Coli growth, we modified non-infectious gram-positive bacteria B.Subtilis by transforming it with a construct, which was designed to produce limulus anti-LPS factor (LALF) together with a signal peptide. LALF is expected to bind and neutralize Lipopolysaccharide (LPS) found in gram-negative bacteria cell wall. On the other hand, to show E.Coli growth inhibition, we prepared another construct, which carries reflectin sequence with a signal peptide sequence and transferred into E.Coli. Reflectin protein produces color by reflecting light in different wavelenghts. This ability helps us to detect whether E.Coli is dead or alive...</p>
 +
 
 +
 +
  <p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">We use two proteins which are anti-LPS (LALF) and Reflectin 1a. Anti-LPS protein can bind to LPS coat of gram negatives. With this function, the protein can be used as protection from septic shock. Also, anti LPS protein works as bacteriostatic.
 +
Reflectin 1a is a camouflage material and the protein is produced by many cephalopods (squid, cuttlefish, and octopus).<br>
 +
In this project, our first aim is to obtain an anti-gram negative bacterial surface with the help of anti-LPS protein. Our second aim is to sense the bacteriostatic effect with colorful reflectin protein. Also, we intend to apply our proteins on a surface as a coat.<br>
 +
We choose <a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Bacillus Subtilis</a> as the host of anti-LPS protein. Because, those bacteria are expected to produce biofilm containing the anti-LPS protein. This ability might help us to apply it on a surface.  </p>
 +
 
 +
<center>
 +
<img src="https://static.igem.org/mediawiki/2011/5/59/Overall1.png"/>
 +
<img src="https://static.igem.org/mediawiki/2011/5/55/Overall2.png.jpg"/>
 +
</center>
 +
 
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">To measure the inhibition effect properly, both of our bacteria are expected to synthesize reflectin. It is planned that anti-LPS exists in the biofilm will bind to LPS on the cell wall of Gram (-) bacteria, thus, Gram (-) bacteria will not grow and survive in our biofilm surface. Around the binding, reflectin thickness is going to decrease and change the color of the surface because of inhibition of growth of E.coli. So, if the color changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.
 +
 
 +
You can see for more information about LALF, Reflectin and Biofilm in the below links:
 +
</p>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px; margin-left:150px" href="https://2011.igem.org/Team:Fatih_Turkey/LALF">
 +
<h2>LALF (Anti-LPS)</h2>
 +
<img src="https://static.igem.org/mediawiki/2011/e/e7/Lalficon.png"/>
 +
</a>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px;" href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">
 +
<h2>Reflectin</h2>
 +
<img src="https://static.igem.org/mediawiki/2011/e/e1/Reflectinicon.png"/>
 +
</a>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px;" href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">
 +
<h2>Biofilm</h2>
 +
<img src="https://static.igem.org/mediawiki/2011/6/62/Biofilmicon.png"/>
 +
</a>
 +
 
 +
</pre>
 +
</div>
 +
    </div>
 +
       </div>
 +
</div>
 +
</div>
 +
 
 +
<div class="footer-sh-wrapper">
 +
<div class="footer-sh-left"></div>
 +
<div class="footer-sh"></div>
 +
<div class="footer-sh-right"></div>
 +
</div>
 +
<div id="footer">
 +
<style>
 +
#footer img:hover{
 +
opacity:0.6;
 +
}
 +
</style>
 +
<div>
 +
<h3 style="color:#BBB;">Sponsors</h3>
 +
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/>
 +
<a href="http://www.sentegen.com">
 +
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a>
 +
</div>
 +
</div>
 +
<div class="copyright-open">
 +
</div>
 +
<div class="copyright">
 +
2011 © Fatih Medical School
 +
</div>
 +
</div>
 +
 
 +
 
</body>
</body>
</html>
</html>

Latest revision as of 20:59, 28 October 2011

deneme baslik

Project

Overall Project

The Rainbow Graveyard


E. Coli is one of the gram-negative bacteria and most of their types are harmless. However some of E.Coli types such as enterohaemorrhagic E.Coli (EHEC) can cause serious diseases.

In our project we designed an innovative model to prevent gram-negative growth and infection. In our model, to prevent E.Coli growth, we modified non-infectious gram-positive bacteria B.Subtilis by transforming it with a construct, which was designed to produce limulus anti-LPS factor (LALF) together with a signal peptide. LALF is expected to bind and neutralize Lipopolysaccharide (LPS) found in gram-negative bacteria cell wall. On the other hand, to show E.Coli growth inhibition, we prepared another construct, which carries reflectin sequence with a signal peptide sequence and transferred into E.Coli. Reflectin protein produces color by reflecting light in different wavelenghts. This ability helps us to detect whether E.Coli is dead or alive...

We use two proteins which are anti-LPS (LALF) and Reflectin 1a. Anti-LPS protein can bind to LPS coat of gram negatives. With this function, the protein can be used as protection from septic shock. Also, anti LPS protein works as bacteriostatic. Reflectin 1a is a camouflage material and the protein is produced by many cephalopods (squid, cuttlefish, and octopus).
In this project, our first aim is to obtain an anti-gram negative bacterial surface with the help of anti-LPS protein. Our second aim is to sense the bacteriostatic effect with colorful reflectin protein. Also, we intend to apply our proteins on a surface as a coat.
We choose Bacillus Subtilis as the host of anti-LPS protein. Because, those bacteria are expected to produce biofilm containing the anti-LPS protein. This ability might help us to apply it on a surface.

To measure the inhibition effect properly, both of our bacteria are expected to synthesize reflectin. It is planned that anti-LPS exists in the biofilm will bind to LPS on the cell wall of Gram (-) bacteria, thus, Gram (-) bacteria will not grow and survive in our biofilm surface. Around the binding, reflectin thickness is going to decrease and change the color of the surface because of inhibition of growth of E.coli. So, if the color changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor. You can see for more information about LALF, Reflectin and Biofilm in the below links:

LALF (Anti-LPS)

Reflectin

Biofilm
2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Project"