Team:Dundee

From 2011.igem.org

(Difference between revisions)
 
(186 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml">
+
<html>
-
<script type="text/javascript" src="https://ajax.googleapis.com/ajax/libs/jqueryui/1.8.12/jquery-ui.min.js"></script>
+
<head>
-
<script type="text/javascript">
+
<link href='http://fonts.googleapis.com/css?family=Droid+Sans' rel='stylesheet' type='text/css'>
-
  function clearHeader() {
+
    <link rel="stylesheet" href="https://2011.igem.org/Team:Dundee/style.css?action=raw&amp;ctype=text/css" />
-
  $('#p-logo').empty();
+
    <link rel="stylesheet" href="https://2011.igem.org/Team:Dundee/sliderstyle.css?action=raw&amp;ctype=text/css" />
-
  $('#top-section').css({
+
    <link rel="stylesheet" href="https://2011.igem.org/Team:Dundee/nivo-slider.css?action=raw&amp;ctype=text/css" />
-
    backgroundImage: 'url(https://static.igem.org/mediawiki/2011/5/5c/Dundee-bg.jpg)'
+
   
-
  });
+
    <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
-
}
+
    <script type="text/javascript" src="https://2011.igem.org/Team:Dundee/cleanPage.js?action=raw&amp;ctype=text/js"></script>
 +
</head>
 +
<body>
 +
    <body>
 +
<div id="wrapper">
-
$(function() {
+
<div id="header">
-
clearHeader();
+
<h1><a href="/Team:Dundee">The University of Dundee</a></h1>
-
})
+
        <div class = "text1">
-
</script>
+
  <h2><span>iGem 2011</span></h2>
-
<head>
+
  </div>
-
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
+
</div>
-
<title>Untitled Document</title>
+
-
<link href="https://dl-web.dropbox.com/get/iGem/twoColElsLt.css?w=e69584a1" />
+
<div id="menu">
-
<!--[if IE]>
+
<ul>
-
<style type="text/css">  
+
-
/* place css fixes for all versions of IE in this conditional comment */
+
<li><a href="/Team:Dundee/Project" title="Project">Project</a></li>  
-
.twoColElsLt #sidebar1 { padding-top: 30px; }
+
<li><a href="/Team:Dundee/Team" title="Team">Team</a></li>
-
.twoColElsLt #mainContent { zoom: 1; padding-top: 15px; }
+
            <li><a href="/Team:Dundee/Modelling" title="Modelling">Modelling</a></li>
-
/* the above proprietary zoom property gives IE the hasLayout it needs to avoid several bugs */
+
            <li><a href="/Team:Dundee/Notebook" title="Notebook">Notebook</a></li>
-
</style>
+
            <li><a href="/Team:Dundee/Results" title="Data">Data</a></li>
-
<![endif]--></head>
+
            <li><a href="/Team:Dundee/Safety" title="Safety">Safety</a></li>
 +
            <li><a href="/Team:Dundee/Sponsors" title="Sponsors">Sponsors</a></li>
 +
            <li ><a href="/Team:Dundee/dnaapp" title="DNA App">DNA App</a></li>
 +
            <li ><a href="/Team:Dundee/Software" title="Software">Software</a></li>
 +
<li ><a href="/Team:Dundee/HumanPractices" title="Human Practices">Human Practices</a></li>
 +
 
-
<body class="twoColElsLt">
+
</ul>
 +
</div>
 +
 +
<div id="container">
 +
<div id="content">
 +
 +
<div class="smallpost">
-
<div id="container">
+
-
  <div id="sidebar1">
+
            <h2><a href="/Team:Dundee/Project">The Sphereactor Project</a></h2>
-
    <ul>
+
<p>Over millennia, eukaryotic cells have evolved sophisticated organelles, which enabled them to partition their cytoplasmic contents into functional sectors (e.g. the nucleus for storage of genetic material).Such compartmentalisation allows greater efficiency of cellular processes,where each organelle is allocated a set of specific metabolic tasks. Some prokaryotes have also developed a method of forming intracellular subdivisions called bacterial microcompartments (BMCs) by producing a set of proteins that ‘cage in’ a reaction pathway to make it more efficient.
 +
One such set of proteins is expressed from the propanediol utilisation (Pdu) operon in Salmonella enterica. Our team aims to design and construct a versatile synthetic microcompartment - The Sphereactor - using genes from the Salmonella pdu operon and an E. coli chassis. In conjunction with new biobricks, and those made by other iGEM groups in the past, we aim to test a universal targeting signal that can be used to pack the Sphereactor with enzymes. This will also help us understand the versatility of our microcompartment and to expand our BMC approach into a wide variety of new applications.</p> 
 +
            </div>
-
  <li><a href="https://2011.igem.org/Team:Dundee" alt="Home Page">Home</a></li>
+
<div class = "imagepost">
 +
<img src="https://static.igem.org/mediawiki/igem.org/3/3a/Dundee-medal-fixed.jpg" alt ="Gold Medal" class="medal" />
 +
<img src = "https://static.igem.org/mediawiki/2011/d/df/Microcompartment1.jpg" alt = "Microcompartment" class="microImage">
 +
</div>
 +
       
 +
<div class="largepost-red">
 +
<h2>Introduction</h2>
 +
<p>In its simplest form, our Sphereactor is a protein shell, that with the addition of enzymes, can do work for us. Whether we want it to clean water of pollutants, degrade a toxic spill or create a new future for softdrinks, we believe our Sphereactor hold the key. And the brilliant thing is that through our experiments, we have shown it to survive outside of the cell, opening up a huge range of possibilities for the use of our sphereactor as a cell free system. This is a foundational advance in the field of synthetic biology, bypassing all concerns for the eventual release of genetically modified bacteria. We have also investigated the targeting of Ferritin to the Sphereactor with the aim of developing a recall system. This application of the sphereactor represents not only a new way to think about synthetic biology in the lab but is also simple way to avoid all issues of competition and horizontal gene transfer upon release.</p>
 +
</div>      
-
  <li><a href="https://2011.igem.org/Team:Dundee/Team" alt="Team Members">Team</a></li>
 
-
 
-
  <li><a href="https://2011.igem.org/Team:Dundee/Project" alt="Project Description">Project</a></li>
 
-
  <li><a href="https://2011.igem.org/Team:Dundee/Notebook" alt="Notebook">Notebook</a></li>
+
              <div class="largepost">
 +
              <h2><a href="/Team:Dundee/Software">Software for All</a></h2>
 +
<p>We are lucky enough to have two Applied Computing students on our team this year. They are focusing on creating software tools and mobile applications to assist the synthetic biologist. </p>
 +
<p>One of tools we are developing right now is a multi-platform application that aims to reduce the need for looking up codon tables and hence speeding up the sequencing process.</p>
 +
<p>As part of our human practices effort, we have created <a href="http://jspnet.computing.dundee.ac.uk/synbin/">The Syn Bin</a> in order to share and discuss lab accidents.</p>
 +
<p>The main aim of our technology team is to provide efficient, usable and super-cool software for all.</p>
 +
   
 +
        </div>
 +
       
 +
       
 +
        <div class="leftpost">
 +
        <h2><a href="/Team:Dundee/Team">The Sphereactor Team</a></h2>
 +
<p>This is the first iGem team to emerge from the University of Dundee. We are all very excited to be taking part in the competition.</p>
 +
                <p>We are a multi-disciplined team made up of students, advisors and supervisors from Applied Computing, Life Sciences and Mathematics. We feel having a mix of these skills will give us a competitive edge and a well-rounded skill set.</p>             
 +
   
 +
            </div>   
 +
 
 +
        <div class="rightpost">
 +
        <h2><a href="/Team:Dundee/Sponsors"">Sphereactor Sponsors</a></h2>
 +
<p>Thanks for the support our sponsors have given us. Without them the project would not have happened.</p>
 +
        </div>
 +
 
 +
        <div class="rightimagepost">
 +
        <a href="/Team:Dundee/Sponsors"><img src="https://static.igem.org/mediawiki/2011/9/96/Sponsors1.jpg" alt ="sponsors logos"></a>
 +
        </div>
 +
 
 +
       
 +
          <div class = "leftimagepost">
 +
        <a href = "https://2011.igem.org/Main_Page"><img src = "https://static.igem.org/mediawiki/2011/6/68/IgemLogo.png" alt = "iGEM Logo"></a>
 +
        </div>
 +
 
 +
 +
 +
 +
</div>
 +
                <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
 +
            <script type="text/javascript">
 +
                $(document).ready(function () {
 +
                    // get the last tweet
 +
                    var username = 'DundeeiGEMTeam';
 +
                    var format = 'json';
 +
                    var url = 'http://api.twitter.com/1/statuses/user_timeline/' + username + '.' + format + '?callback=?'; // make the url
 +
                    $.getJSON(url, function (tweet) { // get the tweets
 +
                        $("#lasttweet").append('"' + tweet[0].text + '"'); // get the first tweet in the response and place it inside the div
 +
                    });
 +
                });
 +
            </script>
 +
           
 +
            <script type="text/javascript">
 +
                $(document).ready(function () {
 +
                    // get the last tweet
 +
                    var username = 'DundeeiGEMTeam';
 +
                    var format = 'json';
 +
                    var url = 'http://api.twitter.com/1/statuses/user_timeline/' + username + '.' + format + '?callback=?'; // make the url
 +
                    $.getJSON(url, function (tweet) { // get the tweets
 +
                        $("#tweetDate").append(tweet[0].created_at); // get the first tweet in the response and place it inside the div
 +
                    });
 +
                });
 +
            </script>
 +
        <img src="https://static.igem.org/mediawiki/2011/b/bd/Twitterblog.png" alt = "twitter bird"/>
 +
      <div id="lasttweet"></div>
 +
 
 +
<div id="footer">
 +
                        <div class = "text2">
 +
      <h2><span><a href="http://www.facebook.com/pages/Dundee-iGem-Team-2011/165447726856145">Find us on Facebook</a></span></h2>
 +
              </div>
 +
              <div class = "text3">
 +
  <h2><span><a href="http://twitter.com/#!/DundeeiGEMTeam">Follow us on Twitter</a></span></h2>
 +
            </div>
 +
</div>
 +
      <div id="lasttweet"></div>
 +
</div>
 +
</div>
-
  <li><a href="https://2011.igem.org/Team:Dundee/Safety" alt="Safety">Safety</a></li>
 
-
</ul>
 
-
  <!-- end #sidebar1 --></div>
 
-
  <div id="mainContent">
 
-
    <h1> Main Content </h1>
 
-
    <p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Praesent aliquam,  justo convallis luctus rutrum, erat nulla fermentum diam, at nonummy quam  ante ac quam. Maecenas urna purus, fermentum id, molestie in, commodo  porttitor, felis. Nam blandit quam ut lacus. </p>
 
-
    <p>Quisque ornare risus quis  ligula. Phasellus tristique purus a augue condimentum adipiscing. Aenean  sagittis. Etiam leo pede, rhoncus venenatis, tristique in, vulputate at,  odio. Donec et ipsum et sapien vehicula nonummy. Suspendisse potenti. Fusce  varius urna id quam. Sed neque mi, varius eget, tincidunt nec, suscipit id,  libero. In eget purus. Vestibulum ut nisl. Donec eu mi sed turpis feugiat  feugiat. Integer turpis arcu, pellentesque eget, cursus et, fermentum ut,  sapien. Fusce metus mi, eleifend sollicitudin, molestie id, varius et, nibh.  Donec nec libero.</p>
 
-
    <h2>H2 level heading </h2>
 
-
    <p>Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Praesent aliquam,  justo convallis luctus rutrum, erat nulla fermentum diam, at nonummy quam  ante ac quam. Maecenas urna purus, fermentum id, molestie in, commodo  porttitor, felis. Nam blandit quam ut lacus. Quisque ornare risus quis  ligula. Phasellus tristique purus a augue condimentum adipiscing. Aenean  sagittis. Etiam leo pede, rhoncus venenatis, tristique in, vulputate at, odio.</p>
 
-
<!-- end #mainContent --></div>
 
-
<!-- This clearing element should immediately follow the #mainContent div in order to force the #container div to contain all child floats --><br class="clearfloat" />
 
-
<!-- end #container --></div>
 
</body>
</body>
 +
</html>
</html>

Latest revision as of 20:20, 28 October 2011

The Sphereactor Project

Over millennia, eukaryotic cells have evolved sophisticated organelles, which enabled them to partition their cytoplasmic contents into functional sectors (e.g. the nucleus for storage of genetic material).Such compartmentalisation allows greater efficiency of cellular processes,where each organelle is allocated a set of specific metabolic tasks. Some prokaryotes have also developed a method of forming intracellular subdivisions called bacterial microcompartments (BMCs) by producing a set of proteins that ‘cage in’ a reaction pathway to make it more efficient. One such set of proteins is expressed from the propanediol utilisation (Pdu) operon in Salmonella enterica. Our team aims to design and construct a versatile synthetic microcompartment - The Sphereactor - using genes from the Salmonella pdu operon and an E. coli chassis. In conjunction with new biobricks, and those made by other iGEM groups in the past, we aim to test a universal targeting signal that can be used to pack the Sphereactor with enzymes. This will also help us understand the versatility of our microcompartment and to expand our BMC approach into a wide variety of new applications.

Gold Medal Microcompartment

Introduction

In its simplest form, our Sphereactor is a protein shell, that with the addition of enzymes, can do work for us. Whether we want it to clean water of pollutants, degrade a toxic spill or create a new future for softdrinks, we believe our Sphereactor hold the key. And the brilliant thing is that through our experiments, we have shown it to survive outside of the cell, opening up a huge range of possibilities for the use of our sphereactor as a cell free system. This is a foundational advance in the field of synthetic biology, bypassing all concerns for the eventual release of genetically modified bacteria. We have also investigated the targeting of Ferritin to the Sphereactor with the aim of developing a recall system. This application of the sphereactor represents not only a new way to think about synthetic biology in the lab but is also simple way to avoid all issues of competition and horizontal gene transfer upon release.

Software for All

We are lucky enough to have two Applied Computing students on our team this year. They are focusing on creating software tools and mobile applications to assist the synthetic biologist.

One of tools we are developing right now is a multi-platform application that aims to reduce the need for looking up codon tables and hence speeding up the sequencing process.

As part of our human practices effort, we have created The Syn Bin in order to share and discuss lab accidents.

The main aim of our technology team is to provide efficient, usable and super-cool software for all.

The Sphereactor Team

This is the first iGem team to emerge from the University of Dundee. We are all very excited to be taking part in the competition.

We are a multi-disciplined team made up of students, advisors and supervisors from Applied Computing, Life Sciences and Mathematics. We feel having a mix of these skills will give us a competitive edge and a well-rounded skill set.

Sphereactor Sponsors

Thanks for the support our sponsors have given us. Without them the project would not have happened.

sponsors logos
iGEM Logo
twitter bird

Retrieved from "http://2011.igem.org/Team:Dundee"