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Copenhagen/11 August 2011
From 2011.igem.org
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* Pcr on the A2 colonies and on an old A1, to check for insert. Both of them are in PSB1C3 | * Pcr on the A2 colonies and on an old A1, to check for insert. Both of them are in PSB1C3 | ||
| - | * TLC on A1 from the membrane preparation from yesterday | + | * [[Team:Copenhagen/Protocol#TLC|TLC]] on A1 from the membrane preparation from yesterday |
| - | * Try the user-cloning assembly from the DTU-team-2 [[Team:Copenhagen/Protocol | + | * Try the user-cloning assembly from the DTU-team-2 with this protocol:[[Team:Copenhagen/Protocol#USER-cloning|USER-cloning]] |
| - | * Grow Bl21 cells with the A2 insert for membrane prepration tommorrow. | + | * [[Team:Copenhagen/Protocol#Expression & Purification in BL21|Grow Bl21 cells]] with the A2 insert for membrane prepration tommorrow. |
Latest revision as of 14:10, 15 August 2011
Wednesday
The A2 coloies we plated out yesterday, to check if they were coloured (no insert), were all white, so hopefully the have the insert.
Our instructor Kenneth is back. Eventhough the absoption spectras from yesterday on A1 didn't give a clear peak at 450 nm the protein can still be active, according to Kenneth. Today we will therefor give the protein substrate and hope that it will produce oximes.
Lab
- Pcr on the A2 colonies and on an old A1, to check for insert. Both of them are in PSB1C3
- TLC on A1 from the membrane preparation from yesterday
- Try the user-cloning assembly from the DTU-team-2 with this protocol:USER-cloning
- Grow Bl21 cells with the A2 insert for membrane prepration tommorrow.
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